Studies on Polynucleotides, C A Novel Joining Reaction Catalyzed by the T4-Polynucleotide Ligase
TLDR
The polynucleotide ligase isolated from T4-infected Escherichia coli is shown to bring about repair of breaks in the single strands of bihelical DNA and can also catalyze the joining of DNA duplexes at their base-paired ends.Abstract:
The polynucleotide ligase isolated from T4-infected Escherichia coli was previously shown to bring about repair of breaks in the single strands of bihelical DNA. The present work shows that the enzyme can also catalyze the joining of DNA duplexes at their base-paired ends. This novel reaction occurs when the deoxynucleoside at a 5′-end carries a phosphate group and the complementary deoxynucleoside opposite to it carries a 3′-hydroxyl group. The consequence is the lengthening of the original duplex to form dimers or oligomers depending upon whether one or both ends are base-paired.read more
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Book ChapterDOI
Enzymatic End-to-end Joining of DNA Molecules
Peter E. Lobban,A.D. Kaiser +1 more
TL;DR: In this article, a way to join naturally occurring DNA molecules, independent of their base sequence, is proposed, based upon the presumed ability of the calf thymus enzyme terminal deoxynucleotidyltransferase to add homopolymer blocks to the ends of double-stranded DNA.
Book ChapterDOI
Synthetic oligodeoxynucleotides for analyses of DNA structure and function.
Ray Wu,Chander P. Bahl +1 more
TL;DR: The chapter presents a general picture of how synthetic oligonucleotides have been used to solve various problems in molecular biology and concludes that, optimal conditions may be found so that large quantities of this RNA transcript would then be used to direct the synthesis in the micro-organism of this protein product in bulk quantities.
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Molecular cloning of DNA. An introduction into techniques and problems.
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