Journal ArticleDOI
Studies on the affinity of methanol--and methane--utilizing bacteria for their carbon substrates.
TLDR
Methanol concentrations of 100 times the Km for formaldehyde so that inhibition by formaldehyde formed as an intermediate in methanol oxidation would be very unlikely and a methane limited chemostat culture of this organism would be expected.Abstract:
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Methanol- and methane-utilizing organisms were grown in chemostat culture and the response of respiration rate to different concentrations of substrate was measured. Cells of Pseudomonas extorquens NCIB 9399 grown on methanol demonstrated a high affinity for methanol (Km=20·4 μM). The affinities for formaldehyde and formate were 104 μM and 228 μM, respectively. The maximum respiration rate was similar for all 3 substrates. Methanol concentrations of 100 times the Km for formaldehyde so that inhibition by formaldehyde formed as an intermediate in methanol oxidation would be very unlikely. The Km for methane of a culture of a pseudomonad grown on methane was very low, 26 μM; that of the same organism for methanol was 50 μM. The maximum respiration on methanol was c. twice that on methane so that no methanol accumulation would be expected in a methane limited chemostat culture of this organism.read more
Citations
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Methane production and methane consumption: a review of processes underlying wetland methane fluxes.
TL;DR: In this article, the potential rates of both methane production and consumption vary over three orders of magnitude and their distribution is skew, and these rates are weakly correlated with ecosystem type, incubation temperature, in situ aeration, latitude, depth and distance to oxic/anoxic interface.
Journal ArticleDOI
Consumption of atmospheric methane in soils of central Panama: Effects of agricultural development
TL;DR: In this paper, in situ measurements of soil methane consumption in a moist forest area of central Panama indicate that the conversion of forests to agricultural lands diminishes the soil sink for atmospheric methane.
Journal ArticleDOI
Spatial and temporal patterns of CH 4 and N 2 O fluxes in terrestrial ecosystems of North America during 1979–2008: application of a global biogeochemistry model
TL;DR: In this article, a process-based global biogeochemical model, the Dynamic Land Ecosystem Model (DLEM), was used to quantify terrestrial CH4 and N2O fluxes in North America's terrestrial ecosystems from 1979 to 2008.
Journal ArticleDOI
Comparison Between Model Simulations and Field Results for In‐Situ Biorestoration of Chlorinated Aliphatics: Part 1. Biostimulation of Methanotrophic Bacteria
Lewis Semprini,Perry L. McCarty +1 more
TL;DR: In this paper, a nonsteady-state model is presented for the in-situ biostimulation of a microbial population in saturated porous media, which includes basic processes of microbial growth, utilization of electron donor and acceptor, advective transport, dispersion, and sorption in porous media.
Journal ArticleDOI
Purification and properties of the methane mono-oxygenase enzyme system from Methylosinus trichosporium OB3b.
TL;DR: A three-component enzyme system that catalyses the oxidation of methane to methanol has been highly purified from Methylosinus trichosporium and the soluble CO-binding cytochrome c shows oxidase acitivity and the relationship between this and the oxygenase activity is discussed.
References
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Journal ArticleDOI
Enrichment, isolation and some properties of methane-utilizing bacteria.
TL;DR: The organisms were classified into five groups on the basis of morphology, fine structure, and type of resting stage formed (exospores and different types of cysts) and into subgroups on other properties.
Journal ArticleDOI
Oxygenation of methane by methane-grown Pseudomonas methanica and Methanomonas methanooxidans
I. J. Higgins,J. R. Quayle +1 more
TL;DR: The results showed that the oxygen in methanol was derived exclusively from gaseous oxygen in both micro-organisms, and control experiments confirmed that there was negligible incorporation of the oxygen atom from water into meethanol.
Journal ArticleDOI
Production of Bacterial Cells from Methane
TL;DR: A mixed methane-oxidizing bacterial culture capable of stable and predictable growth in continuous culture was isolated and results appear to indicate an oxygen to methane mass-transfer coefficient ratio of approximately 1.4.
Journal ArticleDOI
Microbial growth on C1 compounds. Uptake of [14C]formaldehyde and [14C]formate by methane-grown Pseudomonas methanica and determination of the hexose labelling pattern after brief incubation with [14C]methanol.
M. B. Kemp,J. R. Quayle +1 more
TL;DR: The results have been interpreted in terms of a variant of the pentose phosphate cycle, involving the condensation of formaldehyde with C-1 of ribose 5-phosphate to give allulose phosphate.