Open AccessJournal Article
Study on the Isolation and Growth Condition of Methanogen
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Two methanogen strains were isolated from methane fermenting liquor by using Hungate obligate anaerobic technique with methanol,formate and acetate as carbon and energy source and indicated that they were sensitive to the change of pH value and temperature.Abstract:
Two methanogen strains were isolated from methane fermenting liquor by using Hungate obligate anaerobic technique with methanol,formate and acetate as carbon and energy source.They emit blue-green fluorescence when observed by fluorogenic microscope.Their colonies in roll tubes are irregular round and white or a little yellow.In addition,the effect of different pH value and temperature of the growth of the methanogen was studied.The methanogen's optimum growth pH value is 7.0 and can range from 6.5 to 8.0,and the optimum growth temperature is 35 ℃,the results indicated that they were sensitive to the change of pH value and temperature,so a steady circumstance is needed.read more
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