Sulfonylurea-resistant mutants of Arabidopsis thaliana
TLDR
Acetohydroxy acid synthase activity in extracts from chlorsulfuron-resistant plants was much less-susceptible to inhibition by chloursulfuron and a structurally related inhibitor than the activity in wild-type extracts, which suggests that the csr locus is the structural gene for acetohydroxy Acid synthase.Abstract:
Chlorsulfuron-resistant mutants of Arabidopsis thaliana were isolated by screening for growth of seedlings in the presence of the herbicide. Both whole plants and derived tissue cultures were resistant to concentrations of the herbicide approximately 300-fold higher than that required to prevent growth of the wild-type. The resistance is due to a single dominant nuclear mutation at a locus designated csr which has been genetically mapped to chromosome-3. Acetohydroxy acid synthase activity in extracts from chlorsulfuron-resistant plants was much less-susceptible to inhibition by chlorsulfuron and a structurally related inhibitor than the activity in wild-type extracts. This suggests that the csr locus is the structural gene for acetohydroxy acid synthase.read more
Citations
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Auxin: regulation, action, and interaction.
Andrew Woodward,Bonnie Bartel +1 more
TL;DR: Nearly six decades after the structural elucidation of IAA, many aspects of auxin metabolism, transport and signalling are well established; however, more than a few fundamental questions and innumerable details remain unresolved.
Journal ArticleDOI
The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor.
Ruth R. Finkelstein,Tim J. Lynch +1 more
TL;DR: Comparison of seed and ABA-inducible vegetative gene expression in wild-type and abi5-1 plants indicates that ABI5 regulates a subset of late embryogenesis–abundant genes during both developmental stages.
Journal ArticleDOI
A DNA Transformation–Competent Arabidopsis Genomic Library in Agrobacterium
TL;DR: This DNA transformation competent genomic library should expedite gene isolation, by gene rescue, in multicel–lular organisms like Arabidopsis, as with the unicellular organisms Escherichia coli and Saccharomyces cerevisiae.
Journal ArticleDOI
Insensitivity to Ethylene Conferred by a Dominant Mutation in Arabidopsis thaliana
TL;DR: Results of ethylene binding experiments in vivo indicate that this receptor may be affected by the etr mutation, and this receptor appears to share some common element in their transduction pathways—for example, a single receptor for ethylene.
Patent
Herbicide resistance in plants
TL;DR: Products of the invention include plants, plant tissues and seeds which exhibit resistance to such herbicides resulting from expression of genes encoding herbicide resistant acetohydroxyacid synthase enzyme.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Site of action of chlorsulfuron: inhibition of valine and isoleucine biosynthesis in plants.
TL;DR: Addition of these two amino acids to excised pea root cultures incubated in the presence of chlorsulfuron completely alleviates herbicide-induced growth inhibition.
Journal ArticleDOI
Imidazolinones: Potent Inhibitors of Acetohydroxyacid Synthase
TL;DR: The imidazolinones, a new chemical class of herbicides, were shown to be uncompetitive inhibitors of acetohydroxyacid synthase from corn, which is the first common enzyme in the biosynthetic pathway for valine, leucine, and isoleucine.
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