Journal ArticleDOI
Synaptic inputs to broad thorny ganglion cells in macaque retina.
Andrea S. Bordt,Andrea S. Bordt,Sara S. Patterson,Rebecca J. Girresch,Diego Perez,Luke Tseng,James R. Anderson,Marcus Mazzaferri,James A. Kuchenbecker,Rodrigo Gonzales-Rojas,Ashley Roland,Charis Tang,Christian Puller,Alice Z. Chuang,Judith Mosinger Ogilvie,Jay Neitz,David W. Marshak +16 more
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TLDR
A connectome from the central macaque retina was generated by serial block-face scanning electron microscopy, and a broad thorny cell was reconstructed, and its synaptic inputs were analyzed as mentioned in this paper.Abstract:
In primates, broad thorny retinal ganglion cells are highly sensitive to small, moving stimuli. They have tortuous, fine dendrites with many short, spine-like branches that occupy three contiguous strata in the middle of the inner plexiform layer. The neural circuits that generate their responses to moving stimuli are not well-understood, and that was the goal of this study. A connectome from central macaque retina was generated by serial block-face scanning electron microscopy, a broad thorny cell was reconstructed, and its synaptic inputs were analyzed. It received fewer than 2% of its inputs from both ON and OFF types of bipolar cells; the vast majority of its inputs were from amacrine cells. The presynaptic amacrine cells were reconstructed, and seven types were identified based on their characteristic morphology. Two types of narrow-field cells, knotty bistratified type 1 and wavy multistratified type 2, were identified. Two types of medium-field amacrine cells, ON starburst and spiny, were also presynaptic to the broad thorny cell. Three types of wide-field amacrine cells, wiry type 2, stellate wavy and semilunar type 2, also made synapses onto the broad thorny cell. Physiological experiments using a macaque retinal preparation in vitro confirmed that broad thorny cells received robust excitatory input from both the ON and the OFF pathways. Given the paucity of bipolar cell inputs, it is likely that amacrine cells provided much of the excitatory input, in addition to inhibitory input. This article is protected by copyright. All rights reserved.read more
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Journal ArticleDOI
Satb1 expression in retinal ganglion cells of marmosets, macaques, and humans.
Subha Nasir-Ahmad,Kurt A. Vanstone,Mario Novelli,Sammy C.S. Lee,Michael Tri H. Do,Paul R. Martin,Ulrike Grünert +6 more
TL;DR: In this paper, the authors used antibodies against the AT-rich binding protein 1 (Satb1, a protein which is expressed by on-off direction-selective ganglion cells in mouse retina) to study Satb1 expression in the retina of marmosets, macaques, and humans.
Journal ArticleDOI
Conserved circuits for direction selectivity in the primate retina
Sara S. Patterson,Jay Neitz +1 more
TL;DR: In this paper , a connectomic reconstruction of the primate ON SAC circuit from a serial electron microscopy volume of the macaque central retina is presented, showing that the structural basis for the SACs' ability to confer directional selectivity on postsynaptic neurons is conserved.
Journal ArticleDOI
Synaptic inputs to displaced intrinsically-photosensitive ganglion cells in macaque retina
TL;DR: In this paper , three sets of horizontal, ultrathin sections through central macaque retina were collected using serial block-face scanning electron microscopy to investigate their synaptic inputs, and they were identified as the displaced M1 type of ipRGCs based on this morphology and on the high density of granules in their somas.
Journal ArticleDOI
Contribution of parasol-magnocellular pathway ganglion cells to foveal retina in macaque monkey
TL;DR: In this article , the authors used antibodies against carbonic anhydrase 8 (CA8) and intracellular injections of the liphilic dye DiI to show that CA8 selectively labels OFF parasol cells in macaque retina.
Posted ContentDOI
Trogocytosis of neurons and glial cells by microglia in healthy adult macaque retina
TL;DR: In this article , a volume of serial, ultrathin sections of central macaque retina in which many neurons that ramify in the inner plexiform layer (IPL) had been reconstructed previously was analyzed.
References
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Journal ArticleDOI
Kainate receptors mediate synaptic input to transient and sustained OFF visual pathways in primate retina.
Theresa Puthussery,Kumiko A. Percival,Kumiko A. Percival,Kumiko A. Percival,Sowmya Venkataramani,Jacqueline Gayet-Primo,Ulrike Grünert,Ulrike Grünert,W. Rowland Taylor +8 more
TL;DR: The results indicate that transient signaling in the OFF pathway of macaques is not dependent on AMPA receptors and that heterogeneity of KARs and accessory proteins may contribute to the formation of parallel temporal channels.
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Control of aversion by glycine-gated GluN1/GluN3A NMDA receptors in the adult medial habenula.
Yo Otsu,Emmanuel Darcq,K. Pietrajtis,Ferenc Mátyás,Ferenc Mátyás,Eric J Schwartz,T. Bessaih,S. Abi Gerges,Charly V. Rousseau,Teddy Grand,Stéphane Dieudonné,Pierre Paoletti,László Acsády,Cendra Agulhon,Brigitte L. Kieffer,Marco A. Diana +15 more
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Journal ArticleDOI
ON Bipolar Cells in Macaque Retina: Type-Specific Synaptic Connectivity with Special Reference to OFF Counterparts.
Yoshihiko Tsukamoto,Naoko Omi +1 more
TL;DR: SSTEM was used for the rod-dense perifoveal area of macaque retina, reconfirmed ON (cone) bipolar cells to be classified as invaginating midget bipolar (IMB), diffuse bipolar (DB)4, DB5, DB6, giant bipolar (GB), and blue bipolar (BB) types, and clarified their type-specific connectivity.
Journal ArticleDOI
Survey of retinal ganglion cell morphology in marmoset.
TL;DR: The fovea is dominated by midget and parasol cells, but outside the foveA the ganglion cell diversity in marmoset is likely as great as that reported for nonprimate retinas.
Journal ArticleDOI
OFF bipolar cells in macaque retina: type-specific connectivity in the outer and inner synaptic layers.
Yoshihiko Tsukamoto,Naoko Omi +1 more
TL;DR: This paper presents a meta-answers to the question: "What is the best method to assess the likelihood that a person’s immune system is compromised during and after an attack?"