Journal ArticleDOI
Temperature dependence of retention in reversed-phase liquid chromatography. 1. Stationary-phase considerations.
Lynn A. Cole,John G. Dorsey +1 more
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TLDR
Thermodynamic constants for the transfer of a solute from the mobile phase to the stationary phase and comparison of these values to previously reported values indicated that the chromatographic retention process is not well-modeled by bulk-phase oil-water partitioning processes, providing additional support that partitioning is the relevant model of retention.Abstract:
The retention mechanism in reversed-phase liquid chromatography (RPLC) has been examined over a wide temperature range with emphasis on the role of the mobile phase. van't Hoff plot shapes were used to assess the retention mechanism, and the data showed evidence of the hydrophobic effect when water-rich and/or hydrogen-bonded mobile phases such as methanol/water were used. However, different van't Hoff plot shape was observed with acetonitrile/water mobile phases, indicating a change in the retention mechanism. These data showed that the hydrophobic effect, which had previously been proposed as the driving force for retention, is not a satisfactory explanation for the retention process in all RPLC systems.read more
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Journal ArticleDOI
Retention behavior of small polar compounds on polar stationary phases in hydrophilic interaction chromatography.
Yong Guo,Sheetal Gaiki +1 more
TL;DR: The retention of the acids on the amino phase decreased with increasing salt concentration in the mobile phase due to the ion-exchange effect, and the retention process was endothermic as opposed to exothermic on other phases.
Journal ArticleDOI
Hydrophobic interaction chromatography of proteins.
TL;DR: The different proposed theories for the retention mechanism of proteins in HIC are presented and the main parameters to consider for the optimization of fractionation processes by HIC and the stationary phases available were described.
Journal ArticleDOI
Immobilized-artificial-membrane chromatography: measurements of membrane partition coefficient and predicting drug membrane permeability
TL;DR: For 23 structurally unrelated compounds, solute capacity factors measured on IAM columns correlate very well with the solute equilibrium partition coefficients measured in fluid liposome systems (r = 0.907), which indicates that solute partitioning between the IAM bonded phase and the aqueous mobile phase is similar to the solutesome-aqueous phase.
Journal ArticleDOI
Membrane partitioning: Distinguishing bilayer effects from the hydrophobic effect
TL;DR: It is shown through measurements of the heat capacity changes associated with the partitioning of tryptophan side-chain analogs into lipid bilayers and into bulk cyclohexane that the hydrophobic effect plays a crucial role regardless of the large negative enthalpy.
Journal ArticleDOI
Effect of temperature in reversed phase liquid chromatography.
TL;DR: The problem of temperature control for thermostating columns which may represent a significant source of peak broadening is discussed: by taking into account the three main parameters such as heat transfer, pressure drop and band broadening resulting from the preheating tube, suitable rules are set up for a judicious choice of the column internal diameter.
References
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Journal ArticleDOI
Dominant forces in protein folding
TL;DR: The present review aims to provide a reassessment of the factors important for folding in light of current knowledge, including contributions to the free energy of folding arising from electrostatics, hydrogen-bonding and van der Waals interactions, intrinsic propensities, and hydrophobic interactions.
Journal ArticleDOI
Solvophobic interactions in liquid chromatography with nonpolar stationary phases
TL;DR: In this article, the solvophobic theory of solvent interaction with non-polar stationary phases in liquid chromatography is examined on the basis of solvophobia theory and a detailed analysis of the effect of the solvent on this process yields an expression for the capacity factor with essentially no adjustable constants.
Journal ArticleDOI
The molecular mechanism of retention in reversed-phase liquid chromatography
John G. Dorsey,Ken A. Dill +1 more
TL;DR: A review of the current understanding of retention of small molecules in reversed-phase liquid chromatography from both a theoretical and experimental perspective can be found in this paper, where the authors discuss the synthetic methodologies for the preparation of reversed phase stationary phases and deal next with the partitioning processes and stationary-phase structural details.
Journal ArticleDOI
Preparation and Chromatographic Properties of Some Chemically Bonded Phases for Reversed-Phase Liquid Chromatography
Gert E. Berendsen,Leo de Galan +1 more
TL;DR: In this paper, the chromatographic activity of the silica surface is discussed on the basis of TMS-phases with various surface coverages, and it is observed that logarithmic retention data for homologous series and different RPphases-partially taken from the literature-form a set of straight lines with a common intersection point.
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