Journal ArticleDOI
Retention behavior of small polar compounds on polar stationary phases in hydrophilic interaction chromatography.
Yong Guo,Sheetal Gaiki +1 more
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TLDR
The retention of the acids on the amino phase decreased with increasing salt concentration in the mobile phase due to the ion-exchange effect, and the retention process was endothermic as opposed to exothermic on other phases.About:
This article is published in Journal of Chromatography A.The article was published on 2005-05-13. It has received 458 citations till now. The article focuses on the topics: Hydrophilic interaction chromatography.read more
Citations
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Hydrophilic interaction chromatography.
Petrus Hemström,Knut Irgum +1 more
TL;DR: The review attempts to summarize the ongoing discussion on the separation mechanism and gives an overview of the stationary phases used and the applications addressed with this separation mode in LC.
Journal ArticleDOI
Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique
Bogusław Buszewski,Sylwia Noga +1 more
TL;DR: Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases and their applications for separations of polar compounds in complex matrices.
Journal ArticleDOI
Stationary and mobile phases in hydrophilic interaction chromatography: a review
TL;DR: This review deals with recent advances in the development of HILIC phase separation systems with special attention to the properties of stationary phases.
Journal ArticleDOI
Separation and quantitation of water soluble cellular metabolites by hydrophilic interaction chromatography-tandem mass spectrometry.
TL;DR: A liquid chromatography-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method for reliable measurement of 141 metabolites, including components of central carbon, amino acid, and nucleotide metabolism is presented.
Journal ArticleDOI
Electrostatic repulsion hydrophilic interaction chromatography for isocratic separation of charged solutes and selective isolation of phosphopeptides.
TL;DR: Electrostatic repulsion-hydrophilic interaction chromatography affords convenient separations of highly charged peptides that cannot readily be resolved by other means, including peptides, amino acids, and nucleotides.
References
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Practical HPLC method development
TL;DR: In this paper, the authors present a systematic approach to the Reversed-Phase Separation of Regular Samples, using a computer assisted method developed by computer assisted software development.
Journal ArticleDOI
Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds
TL;DR: Hydrophilic-interaction chromatography fractionations resemble those obtained through partitioning mechanisms, and the chromatography of DNA, in particular, resembles the partitioning observed with aqueous two-phase systems based on polyethylene glycol and dextran solutions.
Journal ArticleDOI
Analysis of highly polar compounds of plant origin: Combination of hydrophilic interaction chromatography and electrospray ion trap mass spectrometry
Vladimir Tolstikov,Oliver Fiehn +1 more
TL;DR: In this article, the authors report the separation and analysis of highly polar compounds using hydrophilic interaction liquid chromatography (HILIC) coupled to electrospray ionization (ESI) mass spectrometry.
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Hydrophilic interaction chromatography using amino and silica columns for the determination of polar pharmaceuticals and impurities.
TL;DR: An examination of the retention and separation of several pyrimidines, purines, and amides on silica and amino columns from three manufacturers revealed that mobile phases should contain a buffer or acid for pH control to achieve similar and reproducible results among columns from different sources.
Related Papers (5)
Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds
Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique
Bogusław Buszewski,Sylwia Noga +1 more