Journal ArticleDOI
The chitin-glucan complex ofSaccharomyces cerevisiae
K. Beran,Z. Holan,J. Baldrián +2 more
TLDR
In this paper, the content of glucosamine in the walls of daughter (without bud scars) and mother (multiscar) cells ofSaccharomyces cerevisiae was examined in a control and after treatment with dilute alkali, acid and buffer.Abstract:
The content of glucosamine in the walls of daughter (without bud scars) and mother (multiscar) cells ofSaccharomyces cerevisiae was examined in a control and after treatment with dilute alkali, acid and buffer. The occurrence of chitin in the bud and birth scars is discussed. The results of IR and X-ray analysis of cell-wall fractions indicate the presence of α-chitin which is a part of the chitin-glucan complex. The size of the crystallite of α-chitin in this complex is about 60 A.read more
Citations
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Book ChapterDOI
Fluorescence microscopy methods for yeast.
John R. Pringle,Robert A. Preston,Alison E.M. Adams,Tim Stearns,David G. Drubin,Brian Haarer,Elizabeth W. Jones +6 more
TL;DR: This chapter reviews and provides detailed protocols for the application of immunofluorescence and other fluorescence-microscopic procedures to yeast and shows that these methods are effective with other yeasts such as Schizosaccharomyces pombe and Candida albicans.
Journal ArticleDOI
The chitin system
K. M. Rudall,W. Kenchington +1 more
TL;DR: It is supported that Chitin is not found in Deuterostomia because of the absence of chitin synthetase, and is not find in higher plants because ofThe absence of glucosamine, and in Fungi, control mechanisms are present affecting the synthesis of glucOSamine; chit in is often present, but when it is absent this probably results from a failure to synthesize glucosamines.
Journal ArticleDOI
Patterns of bud-site selection in the yeast Saccharomyces cerevisiae.
John Chant,John R. Pringle +1 more
TL;DR: It is suggested that the distal tip of the cell and each division site carry persistent signals that can direct the selection of a bud site in any subsequent cell cycle.
Book ChapterDOI
Fungal Cell Walls: A Survey
TL;DR: The cell wall is commonly regarded as an assemblage of polymers, mainly polysaccharides, that occurs outside the plasma membrane of cells of plants, fungi, and bacteria that offers protection and, being the outermost cover of cells, often contains molecules involved in interaction between cells.
Journal ArticleDOI
Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae.
TL;DR: It is concluded that damage to the cell wall is caused by excessive chitinase activity at acidic pH, which can normally be repaired through chit in synthesis by Chs1, and the latter emerges as an auxiliary or emergency enzyme.
References
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Journal ArticleDOI
THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY
TL;DR: The stain reported here differs from previous alkaline lead stains in that the chelating agent, citrate, is in sufficient excess to sequester all lead present, and is less likely to contaminate sections.
Journal ArticleDOI
A rapid procedure for the estimation of amino sugars on a micro scale
R. Gatt,E.R. Berman +1 more
Journal ArticleDOI
Specificity of Binding of Hexopyranosyl Polysaccharides with Fluorescent Brightener
Hiroshi Maeda,Nakao Ishida +1 more
TL;DR: Infrared spectral change brought about by the binding might provide a clue to the elucidation of the problems concerning the former two alternatives in order to clarify the nature of binding of the brightener on the polysaccharides.
Related Papers (5)
The location of chitin in the yeast cell wall.
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