The influence of inorganic ions and acclimation salinity on hemocyanin-oxygen binding in the blue crab callinectes sapidus
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The effects of salinity changes on the oxygen binding properties of hemocyanin have been examined in the blue crab Callinectes sapidus and Ca+2 explains only a minor fraction of the actual change in oxygen affinity that accompanies acclimation to a new salinity.Abstract:
The effects of salinity changes on the oxygen binding properties of hemocyanin have been examined in the blue crab Callinectes sapidus. Oxygen affinity increases measurably with increases in Ca+2, Mg+2 or Na+ but, within the physiological range, not K+. Unlike its large and specific influence on other arthropod hemocyanins, Cl- has little or no effect. Ca+2, alone and in physiological concentrations, restores oxygen affinity to the level observed in a complete physiological saline. Ca+2 also restores the Bohr shift to the physiological level. Physiological variation in inorganic ions (or pH) does not affect the cooperativity of oxygen binding.Physiological variation in Ca+2 explains only a minor fraction of the actual change in oxygen affinity that accompanies acclimation to a new salinity. The aclimation, which occurs within 8 days, requires a non-dialyzable factor in the blood. The non-dialyzability of the factor and the previous reports of a large increase in blood protein levels at low salinity might ...read more
Citations
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Functional adaptations of oxygen-transport proteins.
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A comparison of molluscan and arthropod hemocyanin—I. Circular dichroism and absorption spectra
TL;DR: The circular dichroic spectra of molluscan and arthropod oxyhemocyanins are qualitatively and quantitatively different and indicate fundamental differences in the oxygen binding sites of these related proteins.
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Oxygen uptake and the potentiating effects of increased hemolymph lactate on oxygen transport during exercise in the blue crab,Callinectes sapidus
TL;DR: The high hemolymph lactate levels indicate that anaerobic metabolism makes a significant contribution to energy production even during moderate exercise and Callinectes sapidus appears to be highly resistant to fatigue, which correlates with its welldeveloped locomotor capabilities.
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Lactate increases the oxygen affinity of crab hemocyanin
TL;DR: L-lactate increases the oxygen affinity of the hemocyanin in hemolymph dialysates of the crabs Carcinus means and Cancer pagurus without changing the Bohr effect.
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