Journal ArticleDOI
The interaction of plasma proteins with polymers. I. Relationship between polymer surface energy and protein adsorption/desorption
Adam Baszkin,Donald J. Lyman +1 more
TLDR
Results show that the nondispersive-dispersive force balance at the polymer-protein solution interface, expressed by the Ip/WA d ratio, is an important factor for binding of proteins on polymer surfaces.Abstract:
Adsorption of bovine albumin, gamma-globulin and fibrinogen from phosphate buffer solution (pH = 7.5) onto several polymer films was studied using the radioiodinated proteins (125I). The kinetics of desorption of the proteinated polymer films in bovine plasma was determined. Contact angle measurements on these same polymers allowed the calculation of dispersive (WA d) and polar (Ip) components of the polymer-protein solution system. Results from these measurements show that the nondispersive-dispersive force balance at the polymer-protein solution interface, expressed by the Ip/WA d ratio, is an important factor for binding of proteins on polymer surfaces. The purity of fibrinogen and the cleaning procedures for the polymer surfaces influence the absolute values of proteins adsorbed on polymer surfaces.read more
Citations
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Journal ArticleDOI
Mediation of Biomaterial–Cell Interactions by Adsorbed Proteins: A Review
TL;DR: This review illustrates the mediation of cell responses to biomaterials by adsorbed proteins, in the context of osteoblasts and selected materials used in orthopedic implants and bone tissue engineering.
Journal ArticleDOI
Adsorption of proteins from solution at the solid-liquid interface.
TL;DR: Thermodynamic analysis reveals that, under many conditions, the adsorption is driven by an entropy increase that is (partly) related to changes in the structure of the protein molecules.
Journal ArticleDOI
Why do phospholipid polymers reduce protein adsorption
Kazuhiko Ishihara,Hiroto Nomura,Hiroto Nomura,Takashi Mihara,Takashi Mihara,Kimio Kurita,Yasuhiko Iwasaki,Nobuo Nakabayashi +7 more
TL;DR: It is concluded that fewer proteins are adsorbed and their original conformation is not changed on polymer surfaces that possess a high free water fraction.
Journal ArticleDOI
Globular proteins at solid-liquid interfaces.
Charles A. Haynes,Willem Norde +1 more
TL;DR: In this article, the authors take a fresh look at the driving force for protein adsorption by combining recent advances with key results from the past and conclude that four effects, namely structural rearrangements in the protein molecule, dehydration of (parts of) the sorbent surface, redistribution of charged groups in the interfacial layer, and protein surface polarity usually make the primary contributions to the overall adorption behavior.
Journal ArticleDOI
Blood compatible aspects of poly(2-methoxyethylacrylate) (PMEA)--relationship between protein adsorption and platelet adhesion on PMEA surface.
Masaru Tanaka,Tadahiro Motomura,Miho Kawada,Takao Anzai,Yuu Kasori,Toshifumi Shiroya,Kenichi Shimura,Makoto Onishi,Akira Mochizuki +8 more
TL;DR: In this paper, the authors investigated the relationship among the amount of the plasma protein adsorbed onto poly(2-methoxyethylacrylate) (PMEA) and its secondary structure and platelet adhesion.
References
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Journal ArticleDOI
The effect of plasma proteins on the interaction of platelets with glass surfaces.
TL;DR: In this paper, the presence of gelatin in the medium in which washed platelets are suspended decreases the adherence of platelets to surfaces, although it does not diminish the extent of release of platelet contents induced by gamma globulin-coated tubes.
Journal ArticleDOI
Adsorption of plasma proteins in solution to uncharged, hydrophobic polymer surfaces
John L. Brash,Donald J. Lyman +1 more
TL;DR: Infraref internal reflection spectroscopy has been used to study the adsorption of certain plasma proteins on a variety of hydrophobic polymer surfaces, and monolayers are deduced to be closely packed arrays in which the protein molecules appear to retain their native globular form.
Journal ArticleDOI
A surface energy analysis of bioadhesion
D.H. Kaelble,Jovan Moacanin +1 more
TL;DR: In this article, surface energy and fracture mechanics relations were applied to the analysis of bioadhesion and biocompatibility of biomaterials and implant surfaces, showing that a strongly adsorbed plasma protein film on the implant surface provides the best blood compatibility and low thrombogenic effects.
Journal ArticleDOI
The Adsorption and Conformation of Plasma Proteins: a Physical Approach
TL;DR: Although the adsorption of plasma proteins and its role in surface-induced thrombosis are emphasized, many of the techniques described would be equally applicable to investigations of matrix insolubilized enzymes, clinical analysis by antibody flocculation, and cellular adhesion and aggregation.