Journal ArticleDOI
The thermodynamics of calcium binding to thermolysin
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TLDR
Calcium binding to thermolysin is enhanced in the presence of an active site directed inhibitor, suggesting that there may be positive cooperativity between substrate and calcium binding.About:
This article is published in Biophysical Chemistry.The article was published on 1986-03-01. It has received 15 citations till now. The article focuses on the topics: Cooperative binding & Thermolysin.read more
Citations
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Journal ArticleDOI
Prediction of EF-hand calcium-binding proteins and analysis of bacterial EF-hand proteins.
TL;DR: An improvement on the pattern search method for the identification of EF‐hand and EF‐like Ca2+‐binding proteins and a new signature profile has been established to allow for the Identification of pseudo EF‐ hand and S100 proteins from genomic information.
Book ChapterDOI
Determinants that govern high-affinity calcium binding.
Sara Linse,Sture Forsén +1 more
Journal ArticleDOI
Structure-function relationships in EF-hand calcium-binding proteins. Protein engineering and biophysical studies of calbindin D9k
Sara Linse,Peter Brodin,Torbjoern Drakenberg,Eva Thulin,Peter Sellers,Karin Elmdén,Thomas Grundstroem,Sture Forsén +7 more
TL;DR: Genes encoding the minor A component of bovine calbindins D9k--the smallest protein known with a pair of EF-hand calcium-binding sites--with amino acid substitutions and/or deletions have been synthesized and expressed in Escherichia coli and characterized with different biophysical techniques.
Journal ArticleDOI
Acidity, Lipophilicity, Solubility, Absorption, and Polar Surface Area of Some ACE Inhibitors
TL;DR: In this article, the pKa, lipophilicity, solubility, absorption, and polar surface area of the most effective ACE inhibitors for the prevention of myocardial infarction, perindopril and ramipril, were found similar.
ComponentDOI
Calcium binding to thermitase. Crystallographic studies of thermitase at 0, 5, and 100 mM calcium.
TL;DR: The three-dimensional crystal structure of thermitase complexed with eglin-c in the presence of 100 mM calcium has been determined and refined at 2.0-A resolution to a R-factor of 16.8%.
References
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Journal ArticleDOI
Measurement of protein-binding phenomena by gel filtration.
J.P. Hummel,William J. Dreyer +1 more
Journal ArticleDOI
Structure and evolution of calcium-modulated proteins.
TL;DR: The intracellular functions of calcium are best understood in terms of calcium's functioning as a second messenger, and this line of thought leads to a suggestion of the evolutionary reason for the choice of calcium as the sole inorganic second messenger.
Journal ArticleDOI
A nonlinear regression program for small computers
TL;DR: A BASIC computer program for performing weighted nonlinear regression is described and it is shown that the program is useful for the analysis of data conforming to the Michaelis-Menten equation, a single exponential, and to binding equations.
Journal ArticleDOI
The effect of pH on the affinities of enzymes for substrates and inhibitors.
Journal ArticleDOI
Structure of thermolysin refined at 1.6 Å resolution
M.A. Holmes,Brian W. Matthews +1 more
TL;DR: The structure of the thermostable protease thermolysin has been refined by a restrained least-squares procedure and supports the previous assertion that the ther mostable proteins in general is due to a combination of factors which can include hydrophobic interactions, hydrogen bonding, ionic interactions, disulfide linkages, metal binding and other forms of stabilization.
Related Papers (5)
The role of bound calcium ions in thermostable, proteolytic enzymes. II. Studies on thermolysin, the thermostable protease from Bacillus thermoproteolyticus.
Gerrit Voordouw,Rodney S. Roche +1 more