Journal ArticleDOI
Thidiazuron-induced de novo shoot organogenesis on seedlings, etiolated hypocotyls and stem segments of Huang-qin
TLDR
Regeneration protocols developed in this study may provide the basis for improvement of this crop through the identification of medicinally active constituents and eventual development optimized pharmaceutical products.Abstract:
Development of an efficient in vitro propagation system for Huang-qin (Scutellaria baicalensis), a traditional Chinese medicinal plant used in the treatment of a wide range of human ailments, is described. Thidiazuron [TDZ: N-phenyl-N′- (1,2,3-thidiazol-5-ylurea)] effectively induced regeneration on cultured intact seedlings, etiolated hypocotyl explants and sterile stem segments of Huang-qin. Histological examinations of excised hypocotyl or nodal explants revealed that adventitious shoots formed through an intermediate callus. Comparison of TDZ-induced regeneration in the three tissue types indicated that isolation of explants was not essential for optimal regenerative efficiency. Significantly more regenerants formed along hypocotyls of intact seedlings (20 shoots/explant) than were observed on excised hypocotyls (9.7 shoots/explant) indicating that endogenous metabolites produced in adjacent tissues provided resources for the shoot initiation. More than 95% of de novo regenerants formed roots and then intact plantlets under either sterile culture or greenhouse conditions. Regeneration protocols developed in this study may provide the basis for improvement of this crop through the identification of medicinally active constituents and eventual development optimized pharmaceutical products.read more
Citations
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Journal ArticleDOI
Thidiazuron: A multi-dimensional plant growth regulator
TL;DR: Recent advancements in TDZ application in plant sciences are discussed and it is shown that TDZ may modify endogenous plant growth regulators, either directly or indirectly and produce reactions in cell/tissue, necessary for its division/regeneration.
Journal Article
Studies on tissue culture of Chinese medicinal plant resources in Taiwan and their sustainable utilization
Satish Manohar Nalawade,Abhay P. Sagare,Chen-Yue Lee,Chao-Lin Kao,Hsin-Sheng Tsay,Jen-Te Hsiang,Tainan Hsien +6 more
TL;DR: Advanced biotechnological methods of culturing plant cells and tissues should provide new means of conserving and rapidly propagating valuable, rare, and endangered medicinal plants.
Journal ArticleDOI
Identification and quantification of eight flavones in root and shoot tissues of the medicinal plant huang-qin (Scutellaria baicalensis Georgi) using high-performance liquid chromatography with diode array and mass spectrometric detection.
TL;DR: A method of analysis of eight flavones using high-performance liquid chromatography-diode array detection (DAD-mass spectrometry) in root and aerial tissues of the medicinal plant Scutellaria baicalensis was developed and the identity of the analytes was confirmed using retention time, UV-vis and mass spectral comparisons to commercial standards.
Journal ArticleDOI
Regeneration of the Egyptian medicinal plant Artemisia judaica L.
TL;DR: The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of A. judaica.
Journal ArticleDOI
A metabolomic analysis of medicinal diversity in Huang-qin (Scutellaria baicalensis Georgi) genotypes: discovery of novel compounds
TL;DR: In vitro manipulation of plant regeneration in the Chinese medicinal species Scutellaria baicalensis Georgi (Huang-qin) resulted in 26 chemically distinct germplasm lines, providing new insights into the biochemical complexity of an important medicinal species and demonstrating the power of in vitro manipulation in combination with untargeted metabolomic screening for the production of new germplasms.
References
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A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
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Journal Article
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Journal ArticleDOI
Thidiazuron : A potent regulator of in vitro plant morphogenesis
TL;DR: Several reports indicate that TDZ may act through modulation of the endogenous plant growth regulators, either directly or as a result of induced stress, and several possibilities include the modification in cell membranes, energy levels, nutrient uptake, or nutrient assimilation.
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