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Book ChapterDOI

Tracking Red Blood Cells Flowing through a Microchannel with a Hyperbolic Contraction: An Automatic Method

B. Taboada, +2 more
- pp 105-119
TLDR
In this article, a microchannel containing a hyperbolic contraction was fabricated in polydimethylsiloxane by using a soft-lithography technique and the images were captured by a standard high-speed microscopy system.
Abstract
The present chapter aims to assess the motion and deformation index of red blood cells (RBCs) flowing through a microchannel with a hyperbolic contraction using an image analysis based method. For this purpose, a microchannel containing a hyperbolic contraction was fabricated in polydimethylsiloxane by using a soft-lithography technique and the images were captured by a standard high-speed microscopy system. An automatic image processing and analyzing method has been developed in a MATLAB environment, not only to track both healthy and exposed RBCs motion but also to measure the deformation index along the microchannel. The keyhole model has proved to be a promising technique to track automatically healthy and exposed RBCs flowing in this kind of microchannels.

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Journal ArticleDOI

Cell-free layer measurements of in vitro blood flow in a microfluidic network: an automatic and manual approach

TL;DR: The present study shows the CFL layer variation at a microchannel network and visualises the in vitro blood flowing through the network and to identify several flow phenomena that happen in microcirculation.
References
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Proceedings Article

An iterative image registration technique with an application to stereo vision

TL;DR: In this paper, the spatial intensity gradient of the images is used to find a good match using a type of Newton-Raphson iteration, which can be generalized to handle rotation, scaling and shearing.
Journal ArticleDOI

Determining optical flow

TL;DR: In this paper, a method for finding the optical flow pattern is presented which assumes that the apparent velocity of the brightness pattern varies smoothly almost everywhere in the image, and an iterative implementation is shown which successfully computes the Optical Flow for a number of synthetic image sequences.
Proceedings ArticleDOI

Bilateral filtering for gray and color images

TL;DR: In contrast with filters that operate on the three bands of a color image separately, a bilateral filter can enforce the perceptual metric underlying the CIE-Lab color space, and smooth colors and preserve edges in a way that is tuned to human perception.
Proceedings ArticleDOI

Determining Optical Flow

TL;DR: In this article, a method for finding the optical flow pattern is presented which assumes that the apparent velocity of the brightness pattern varies smoothly almost everywhere in the image, and an iterative implementation is shown which successfully computes the Optical Flow for a number of synthetic image sequences.
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