Transmissible Plasmid Coding Early Enzymes of Naphthalene Oxidation in Pseudomonas putida
N. W. Dunn,I. C. Gunsalus +1 more
TLDR
The capacity of Pseudomonas putida PpG7 to grow on naphthalene, phenotype Nah(+), is lost spontaneously, and the frequency is increased by treatment with mitomycin C, and this character can be transferred to cured or heterologous fluorescent pseudomonads lacking this capacity by conjugation.Abstract:
The capacity of Pseudomonas putida PpG7 (ATCC 17,485) to grow on naphthalene, phenotype Nah+, is lost spontaneously, and the frequency is increased by treatment with mitomycin C. The Nah+ growth character can be transferred to cured or heterologous fluorescent pseudomonads lacking this capacity by conjugation, or between phage pf16-sensitive strains by transduction. After mutagenesis, strains can be selected with increased donor capacity in conjugation. Clones which use naphthalene grow on salicylate and carry catechol 2,3-oxygenase, the initial enzyme of the aromatic α-keto acid pathway, whereas cured strains grow neither on salicylate nor naphthalene and lack catechol 2,3-oxygenase, but retain catechol 1,2-oxygenase and the aromatic β-keto adipate pathway enzymes.read more
Citations
More filters
Journal ArticleDOI
Biodegradation of polycyclic aromatic hydrocarbons
TL;DR: In this paper, the authors provide an outline of the microbial degradation of polycyclic aromatic hydrocarbons, including bacteria, fungi and algae, and the biochemical principles underlying the degradation.
Journal ArticleDOI
Microbial degradation of petroleum hydrocarbons: an environmental perspective.
Journal ArticleDOI
Bioremediation of high molecular weight polycyclic aromatic hydrocarbons: a review of the microbial degradation of benzo[a]pyrene.
Albert L. Juhasz,Ravendra Naidu +1 more
TL;DR: The focuss of this review is on the high molecular weight PAH benzo[a]pyrene (BaP), which has been observed to accumulate in marine organisms and plants which could indirectly cause human exposure through food consumption.
Journal ArticleDOI
Biodegradation of polycyclic aromatic hydrocarbons
TL;DR: The intent of this review is to provide an outline of the microbial degradation of polycyclic aromatic hydrocarbons, a catabolically diverse microbial community, consisting of bacteria, fungi and algae, metabolizes aromatic compounds.
Journal ArticleDOI
The biodegradation of aromatic hydrocarbons by bacteria
TL;DR: The biodegradation of benzene, certain arenes, biphenyl and selected fused aromatic hydrocarbons, by single bacterial isolates, are dealt with in detail.
References
More filters
Journal ArticleDOI
The Aerobic Pseudomonads a Taxonomic Study
TL;DR: A collection of 267 strains, representing many of the principal biotypes among aerobic pseudomonads, has been subjected to detailed study, with particular emphasis on biochemical, physiological and nutritional characters.
Journal ArticleDOI
Transduction of linked genetic characters of the host by bacteriophage P1
TL;DR: Transduction of characters between bacteria of the coli and dysentery groups indicates genetic homologies between these groups.
Journal ArticleDOI
Synthesis of the Enzymes of the Mandelate Pathway by Pseudomonas putida I. Synthesis of Enzymes by the Wild Type
TL;DR: Dl-mandelate elicits immediate synthesis at a steady rate of the first two enzymes of the pathway, but two enzymes which act below the level of benzoate are synthesized only after a considerable lag, and Succinate and asparagine do not significantly repress the synthesis of the enzymes responsible for mandelate oxidation.
Journal ArticleDOI
Phenol and benzoate metabolism by Pseudomonas putida: regulation of tangential pathways.
Carol F. Feist,G. D. Hegeman +1 more
TL;DR: It is possible to explain the mutually exclusive occurrence of the meta and ortho pathway enzymes in phenol- and benzoate-grown cells of P. putida on the basis of differences in the mode of regulation of these two pathways.
Related Papers (5)
Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid.
Peter A. Williams,Keith Murray +1 more