Ultradian rhythm in the intestine of Caenorhabditis elegans is controlled by the C‐terminal region of the FLR‐1 ion channel and the hydrophobic domain of the FLR‐4 protein kinase
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It is shown that a truncated FLR‐1 lacking the C‐terminal intracellular region resulted in longer periods, suggesting that this region is involved in the negative regulation of defecation cycle periods.Abstract:
Defecation behavior in Caenorhabditis elegans is driven by an endogenous ultradian clock in the intestine. Its periods are positively regulated by FLR-1, an ion channel of the epithelial sodium channel/degenerin superfamily, and FLR-4, a protein kinase with a hydrophobic domain at the carboxyl terminus. FLR-1 has many putative phosphorylation sites in the C-terminal intracellular region. This structure implies that the periods may be regulated by the phosphorylation of FLR-1 by FLR-4, but it remains to be clarified. Here, we show that a truncated FLR-1 lacking the C-terminal intracellular region resulted in longer periods, suggesting that this region is involved in the negative regulation of defecation cycle periods. Contrary to our expectation, FLR-4 was still necessary for the function of the truncated FLR-1. Furthermore, FLR-4 containing a kinase-dead mutation or lacking the whole kinase domain was sufficient for normal defecation cycle periods. FLR-4 was necessary for the stable expression of FLR-1::GFP, and its hydrophobic domain was sufficient also for this function. FLR-1 and FLR-4 are often colocalized in the plasma membrane. These data showed an unexpected role of FLR-4: its hydrophobic domain stabilizes the FLR-1 ion channel, a key regulator of defecation cycle periods in the intestine.read more
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An N-myristoylated globin with a redox-sensing function that regulates the defecation cycle in Caenorhabditis elegans.
L. Tilleman,Sasha De Henau,Martje Pauwels,Nóra Veronika Nagy,Nóra Veronika Nagy,Isabel Pintelon,Bart P. Braeckman,Karolien De Wael,Sabine Van Doorslaer,Dirk Adriaensen,Jean-Pierre Timmermans,Luc Moens,Sylvia Dewilde +12 more
TL;DR: This work proposes a function for a membrane-bound globin of C. elegans, GLB-26, predicted to be myristoylated at its N-terminus, a post-translational modification only recently described in the globin family.
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Distinct roles for two Caenorhabditis elegans acid-sensing ion channels in an ultradian clock
TL;DR: Kaulich et al. as discussed by the authors identified two acid-sensing ion channels, with very different proton sensing properties, and described their role in an ultradian clock, the defecation motor program (DMP) of the nematode Caenorhabditis elegans.
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The Doubletime Homolog KIN-20 Mainly Regulates let-7 Independently of Its Effects on the Period Homolog LIN-42 in Caenorhabditis elegans .
Kyle Rhodehouse,Katherine Cascino,Laura Aseltine,Allegra Padula,Rachel Weinstein,Joseph S Spina,Christiane E Olivero,Priscilla M. Van Wynsberghe +7 more
TL;DR: It is found that kin-20 RNAi enhances loss-of-function lin-42 mutant phenotypes and that Kin-20 mutant worms express lower levels of LIN-42 and let-7 microRNAs, and it is suggested that thoughKin-20 regulates lin-41 and let's7 microRNA, it mainly affects let- 7 microRNA expression independently of lin- 42.
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Distinct roles for two Caenorhabditis elegans acid-sensing ion channels in an ultradian clock
TL;DR: In this article, the authors identify two acid-sensing ion channels, with very different proton sensing properties, and describe their role in an ultradian clock, the defecation motor program (DMP) of the nematode Caenorhabditis elegans.
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Follicle stimulating hormone signaling opposes the DRL-1/FLR-4 MAP Kinases to balance p38-mediated growth and lipid homeostasis in C. elegans
TL;DR: In this paper , a forward genetic screen for suppressors of the drl-1 mutant phenotypes and identified mutations in flr-2 and fshr-1, which encode the orthologues of follicle stimulating hormone and its putative G protein-coupled receptor, respectively.
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