Journal ArticleDOI
Ultrastructure of growth cones formed by isolated rat adrenal medullary chromaffin cells in vitro after treatment with nerve growth factor.
Thomas J. Millar,Klaus Unsicker +1 more
TLDR
Growth cones formed by adrenal chromaffin cells from young postnatal rats cultured in the presence of nerve growth factor were studied at an electron microscopic level supporting the view that NGF-treated chromAffin cells may undergo neuronal transdifferentiation.About:
This article is published in Developmental Brain Research.The article was published on 1981-11-01. It has received 17 citations till now. The article focuses on the topics: Nerve growth factor & Transdifferentiation.read more
Citations
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Journal ArticleDOI
Use of isolated chromaffin cells to study basic release mechanisms
TL;DR: The demonstration of SP action and SP receptors on the chromaffin cells suggests a physiological role for SP in the regulation of secretion from the adrenal medulla, which is contained in the splanchnic nerve terminals that innervate the medulla.
Journal ArticleDOI
Neuronotrophic and neurite-promoting factors: effects on early postnatal chromaffin cells from rat adrenal medulla
TL;DR: The effects on chromaffin cell survival and neurite outgrowth of NGF, ciliary neuronotrophic factor (CNTF), activities contained in various types of conditioned media (CM), and various substrata (laminin, fibronectin and polyornithine-binding neurite-promoting factor from RN 22 Schwannoma cells - PNPF) are quantitated.
Journal ArticleDOI
Cell and tissue culture of the central nervous system: Recent developments and current applications
TL;DR: A survey of methods for cell and tissue culture of the central nervous system (CNS) is given, including a brief historical outline and description of methods in current use.
Journal ArticleDOI
Useful cell lines derived from the adrenal medulla.
M.J. Eaton,H. Duplan +1 more
TL;DR: The strategy of immortalization with conditionally expressed oncogenes has been expanded recently to create the first disimmortalizable chromaffin cells, with an excisable oncogenic cassette, as might be envisioned for the creation of human Chromaffin cell lines.
Journal ArticleDOI
Immunohistochemical and histochemical characterisation of epithelial cells of rabbit lacrimal glands in tissue sections and cell cultures
T.J. Millar,T.J. Millar,George H. Herok,George H. Herok,H. Koutavas,H. Koutavas,Donald K. Martin,Donald K. Martin,Philip J. Anderton +8 more
TL;DR: It is demonstrated that acinar cells from the Harderian and lacrimal glands of rabbit can be isolated and maintained in culture for 20 to 30 days, and that despite dramatic morphological changes, these cells retain their distinctive phenotype as indicated by antibody staining to specific cellular structural proteins such as cytokeratins and actin.
References
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Journal ArticleDOI
THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY
TL;DR: The stain reported here differs from previous alkaline lead stains in that the chelating agent, citrate, is in sufficient excess to sequester all lead present, and is less likely to contaminate sections.
Journal ArticleDOI
The nerve growth factor: purification as a 30,000-molecular-weight protein
TL;DR: The nerve growth factor protein was purified over 100-fold from adult mouse salivary glands by a gel filtration on Sephadex G-100 at pH 7.5 and the over-all recovery was about 45 per cent.
Journal ArticleDOI
Ultrastructure and function of growth cones and axons of cultured nerve cells
TL;DR: Dorsal root ganglion nerve cells undergoing axon elongation in vitro have been analyzed ultrastructurally and it is suggested that this filamentous network could provide the structural basis for the initiation of lateral microspikes and perhaps of collateral axons, besides playing a role in axonal transport.
Journal ArticleDOI
A methodological approach to rapid and sensitive monoamine histofluorescence using a modified glyoxylic acid technique: the SPG method.
J C Torre,J W Surgeon +1 more
TL;DR: The SPG method retains the high specific sensitivity for monoamines previously described in the original technique but is, in addition, more rapid and simple and is easily accessible as a research tool to investigators inexperienced in histofluorescence techniques.
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