Journal ArticleDOI
Use of capillary zone electrophoresis for fish species identification. Differentiation of flatfish species
TLDR
A capillary zone electrophoresis method is described for the analysis of fish sarcoplasmic proteins at low pH; the CZE protein profiles that resulted were specific for each species and could be employed for differentiation and identification purposes.Abstract:
A capillary zone electrophoresis (CZE) method is described for the analysis of fish sarcoplasmic proteins at low pH. Aqueous extracts of eight flatfish species, containing the sarcoplasmic proteins, were subjected to the action of an electric field in a capillary electrophoresis apparatus ; the separated proteins were detected by means of an UV detector after less than 35 min. Acceptable resolution and reproducibility were obtained using low protein concentration (0.1 mg/mL) in the extracts analyzed. The CZE protein profiles that resulted were specific for each species and could be employed for differentiation and identification purposes.read more
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Journal ArticleDOI
Challenges in the identification of species of canned fish
Ian M. Mackie,Susan E. Pryde,C. Gonzales-Sotelo,Isabel Medina,Ricardo I. Pérez-Martín,Javier Quinteiro,Manuel Rey-Méndez,Hartmut Rehbein +7 more
TL;DR: The identification of fish species becomes a problem when the usual identifying characteristics are removed on processing and only a portion of flesh is available and DNA is also degraded.
Journal ArticleDOI
Use of restriction fragment length polymorphism to distinguish between salmon species
Valerie J. Russell,Georgina L. Hold,Susan E. Pryde,Hartmut Rehbein,Javier Quinteiro,Manuel Rey-Méndez,Carmen G. Sotelo,Ricardo I. Pérez-Martín,and Ana T. Santos,Carla Rosa +9 more
TL;DR: Results indicate that it is possible to differentiate between all 10 salmon species and that the technique could be easily adopted by the food industry for analysis of processed salmon products.
Journal ArticleDOI
Characterization and partial sequencing of species-specific sarcoplasmic polypeptides from commercial hake species by mass spectrometry following two-dimensional electrophoresis.
TL;DR: The potential of proteomics was employed in this study with the aim of achieving the differential characterization of five different hake species, revealing a high homology with rat nucleoside diphosphate kinase A (NDKA).
Journal ArticleDOI
Identification of commercial hake and grenadier species by proteomic analysis of the parvalbumin fraction.
TL;DR: Analysis of parvalbumin fractions through proteomic methodologies allowed the differential classification of ten commercial, closely related species of the family Merlucciidae, making genus differentiation possible.
Journal ArticleDOI
Development of a sodium dodecyl sulfate‐polyacrylamide gel electrophoresis reference method for the analysis and identification of fish species in raw and heat‐processed samples: A collaborative study
Carmen Piñeiro,Jorge Barros-Velázquez,Ricardo I. Pérez-Martín,Iciar Martinez,Tonna Jacobsen,Hartmut Rehbein,Rainer Kündiger,Rogério Mendes,Monique Etienne,Marc Jérôme,Anne Craig,Ian M. Mackie,Fleming Jessen +12 more
TL;DR: All the fish species tested, either raw and cooked, yielded reproducible and discriminant species‐specific protein patterns, allowing a better matching of results among the laboratories participating in this collaborative study.