Variation of refractive index of solutions with wavelength.
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TLDR
A simpler and more fundamental derivation can be obtained by considering the way in which the refractive index varies with concentration.Abstract:
Chaubal, Lodin and Korgaonkar (3) and Chaubal, Lodin and Pilny (4) have rightly stated that the development of two-wavelength methods of interference microscopy requires an accurate knowledge of the refractive indices of both the medium and the specimen at different wavelengths. In a recent note (3) they have attempted to derive formulae expressing the variation of the refractive index of protoplasm with wavelength. Their derivation is somewhat cumbersome and includes an assumed value of 100 m i for the optical path difference of a typical cell. Since the optical path difference (which is related to dry mass per unit area) is itself the product of concentration and thickness it rests on two independent variables. A simpler and more fundamental derivation can be obtained by considering the way in which the refractive index varies with concentration. According to the well known relationship (1, 2)read more
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Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium
Benjamin Rappaz,Florian Charrière,Christian Depeursinge,Pierre J. Magistretti,Pierre Marquet +4 more
TL;DR: This Letter presents a dual-wavelength DHM that allows us to separately measure both the RI and the cellular thickness by exploiting an enhanced dispersion of the perfusion medium achieved by the utilization of an extracellular dye.
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Single-shot quantitative dispersion phase microscopy
TL;DR: The technique permits wide-field measurement of the optical phase shifts introduced by a sample for multiple discrete wavelengths in a single image capture, and using spatial modulation and the wavelength dependence of the interference-fringe spacing, average refractive index as a function of wavelength is obtained.
Proceedings ArticleDOI
Simultaneous cell morphometry and refractive index measurement with dual-wavelength Digital Holographic Microscopy
Benjamin Rappaz,Florian Charrière,Tristan Colomb,Christian Depeursinge,Pierre J. Magistretti,Pierre Marquet +5 more
TL;DR: In this paper, the authors measured the refractive index and cellular thickness information contained in the phase signal recorded by Digital Holographic Microscopy (DHM) with a dual-wavelength technique exploiting the dispersion of a dye present in the perfusion medium.
Shot noise, refractive index tomography and aberrations estimation in digital holographic microscopy
TL;DR: In this article, the effect of shot noise on the phase image accuracy, the use of DHM in micro-tomography and in aberrations evaluation of a microscope objective (MO) is discussed.
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Study of optical properties of SnO2 thin films and its influence on gas sensing response
TL;DR: In this paper , optical properties of six SnO2 thin films of varying thickness have been done which are correlated for a deeper understanding of gas sensing phenomenon, the lowest value of refractive index and packing density for 90 nm SnO 2 film substantiate the presence of voids and defects in the film.
References
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Journal ArticleDOI
The specific refractive increment of some purified proteins.
Journal ArticleDOI
Refractometry of Living Cells Part I. Basic Principles
TL;DR: Since living cells contain substances other than proteins the specific refraction increments of protein derivatives, lipides, carbohydrates, and salts are considered and it is shown that the presence of moderate amounts of such substances is unlikely to affect the refractive index of cells to any great extent.
Journal Article
Refractometry of Living Cells
TL;DR: It is shown that the numerical results can be used to obtain the concentration of solids, concentration of water, total wet mass and density, and concentrations in terms of wet weight of tissue.