Journal ArticleDOI
Viral load of human bocavirus-1 in stools from children with viral diarrhoea in Paraguay.
José Luiz Proença-Módena,Magaly Martínez,Alberto A. Amarilla,Emilio E. Espínola,Maria E. Galeano,Norma Fariña,Graciela Russomando,Victor Hugo Aquino,Gabriel I. Parra,Eurico Arruda +9 more
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TLDR
Although HBoV-1 was frequently detected in patients with acute non-bacterial gastroenteritis, there is no clear causal association of this agent with diarrhoea.Abstract:
Since their discovery, four species of human bocavirus (HBoV) have been described in patients with respiratory and gastrointestinal diseases. However, a clear causal association between HBoV-1 and gastroenteritis has not been demonstrated. In this study, we describe the detection and quantification of HBoV-1 in stools from children with acute non-bacterial gastroenteritis using quantitative polymerase chain reaction. HBoV-1 genome was detected in 10·6% of stools with frequent association with rotavirus and norovirus. The median of HBoV-1 viral load was 1·88 × 104 genome/ml, lower than previously shown in secretions of patients with respiratory infections, without any obvious association between high viral load and presence of HBoV as single agent. Thus, although HBoV-1 was frequently detected in these patients, there is no clear causal association of this agent with diarrhoea. Indeed, HBoV-1 DNA in stools of patients with gastroenteritis without respiratory symptoms may be a remnant of previous infections or associated with prolonged shedding of virus in the respiratory or digestive tracts.read more
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Human bocavirus: Current knowledge and future challenges
Marcello Guido,Maria Rosaria Tumolo,Tiziano Verri,Alessandro Romano,Francesca Di Serio,Mattia De Giorgi,Antonella De Donno,Francesco Bagordo,Antonella Zizza +8 more
TL;DR: Current diagnostic methods to support HBoV detection include polymerase chain reaction, real-time PCR, enzyme-linked immunosorbent assay and enzyme immunoassay using recombinant VP2 or virus-like particle capsid proteins, although sequence-independent amplification techniques combined with next-generation sequencing platforms promise rapid and simultaneous detection of the pathogens in the future.
Journal ArticleDOI
Management strategies in the treatment of neonatal and pediatric gastroenteritis.
TL;DR: EU guidelines make a stronger recommendation for the use of probiotics for the management of acute gastroenteritis, particularly those with documented efficacy such as Lactobacillus rhamnosus GG, LactOBacillus reuteri, and Saccharomyces boulardii.
Journal ArticleDOI
Quito's virome: Metagenomic analysis of viral diversity in urban streams of Ecuador's capital city
Laura Guerrero-Latorre,Brigette Romero,Edison Bonifaz,Natàlia Timoneda,Marta Rusiñol,Rosina Girones,Blanca Ríos-Touma +6 more
TL;DR: The wide diversity of species detected through metagenomics gives key information about the public health risks present in the urban rivers of Quito and describes for the first time the presence of important infectious agents not previously reported in Ecuador and with very little reports in Latin America.
Journal ArticleDOI
Relative Abundance of Human Bocaviruses in Urban Sewage in Greater Cairo, Egypt
TL;DR: The high incidence of HBoV in sewage samples provided an evidence of its circulation in the local population and the risk of infection via contaminated water should be taken into consideration.
References
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MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods
Koichiro Tamura,Daniel S. Peterson,Nicholas Peterson,Glen Stecher,Masatoshi Nei,Sudhir Kumar +5 more
TL;DR: The newest addition in MEGA5 is a collection of maximum likelihood (ML) analyses for inferring evolutionary trees, selecting best-fit substitution models, inferring ancestral states and sequences, and estimating evolutionary rates site-by-site.
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Bioedit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/ nt
Journal ArticleDOI
Clustal W and Clustal X version 2.0
Mark A. Larkin,Gordon Blackshields,Nigel P. Brown,R. Chenna,Paul A. McGettigan,Hamish McWilliam,Franck Valentin,Iain M. Wallace,Andreas Wilm,Rodrigo Lopez,J.D. Thompson,Toby J. Gibson,Desmond G. Higgins +12 more
TL;DR: The Clustal W and ClUSTal X multiple sequence alignment programs have been completely rewritten in C++ to facilitate the further development of the alignment algorithms in the future and has allowed proper porting of the programs to the latest versions of Linux, Macintosh and Windows operating systems.
Journal ArticleDOI
Cloning of a human parvovirus by molecular screening of respiratory tract samples
Tobias Allander,Martti T. Tammi,Margareta Eriksson,Annelie Bjerkner,Annika Tiveljung-Lindell,Björn Andersson +5 more
TL;DR: It is suggested that a systematic exploration of the viruses that infect humans, "the human virome," can be initiated, and a general culture-independent solution to the problem of detecting unknown virus species in single or pooled samples is provided.
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Human Bocaviruses Are Highly Diverse, Dispersed, Recombination Prone, and Prevalent in Enteric Infections
Amit Kapoor,Peter Simmonds,Elizabeth Slikas,Elizabeth Slikas,Linlin Li,Linlin Li,Ladaporn Bodhidatta,Orntipa Sethabutr,Henda Triki,Olfa Bahri,Bamidele Soji Oderinde,Marycelin Baba,David Bukbuk,John M. Besser,Joanne M. Bartkus,Eric Delwart,Eric Delwart +16 more
TL;DR: The high degree of genetic diversity seen among the human bocaviruses found in feces specimens, relative to the highly homogeneous HBoV1, suggest that this worldwide-distributed respiratory pathogen may have recently evolved from an enteric bocvirus after acquiring an expanded tropism favoring the respiratory tract.