Virus-like particles associated with the double-stranded RNA species found in killer and sensitive strains of the yeast Saccharomyces cerevisiae.
A. J. Herring,E. A. Bevan +1 more
TLDR
Cell fractionation experiments utilizing strains of the latter type have shown that the dsRNA is associated with isometric virus-like particles similar to those found in other fungi.Abstract:
Summary
Two high mol. wt. double stranded RNA species have been discovered in yeast; some strains possess both species (killers) whilst other strains possess only one. Strains of the latter type have been isolated which possess relatively large amounts of dsRNA. Cell fractionation experiments utilizing these strains have shown that the dsRNA is associated with isometric virus-like particles similar to those found in other fungi. Similar particles, containing both species of dsRNA, have been isolated from a killer strain. The two species of dsRNA appear to be separately encapsidated.read more
Citations
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Journal ArticleDOI
Yeast killer systems.
TL;DR: In this review, the biology, ecology, epidemiology, therapeutics, serology, and idiotypy of yeast killer systems are discussed.
Journal ArticleDOI
Yeast viral killer toxins: lethality and self-protection
Manfred Schmitt,Frank Breinig +1 more
TL;DR: This review focuses on the most recent advances in the understanding of the basic biology of virus-carrying killer yeasts, in particular the toxin-encoding killer viruses, and the intracellular processing, maturation and toxicity of the viral protein toxins.
Journal ArticleDOI
Double-stranded ribonucleic acid killer systems in yeasts.
Book ChapterDOI
The Double-Stranded RNA Viruses of Saccharomyces Cerevisiae
TL;DR: The author revealed that Gag-Pol Fusion Protein Formed by a 21 Ribosomal Frameshift is Necessary for Assembly and evidence for cis Packaging by L-A is found.
Journal ArticleDOI
Chromosomal superkiller mutants of Saccharomyces cerevisiae.
A Toh-E,P Guerry,R B Wickner +2 more
TL;DR: It is found that recessive mutations in any of four chromosomal genes result in the superkiller phenotype, i.e., increased secretion of killer toxin activity by strains carrying the killer genome.
References
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Journal ArticleDOI
The inheritance of the killer character in yeast.
J. M. Somers,E. A. Bevan +1 more
TL;DR: The results of this analysis indicated that the killer character is under the control of two types of cytoplasmic genetic determinant, one of which determines killing, and the other, (n), neutrality.
Journal ArticleDOI
Viruses with double-stranded RNA genomes
TL;DR: The purpose of this article is to present the salient chemical, physical and biological properties of these viruses as currently described, and to discuss the similarities and dissimilarities both within and between groups ofThese viruses.
Journal ArticleDOI
A new species of double-stranded RNA from yeast.
E. A. Berry,E. A. Bevan +1 more
TL;DR: A new species of double-stranded RNA has been detected in certain strains of bakers' yeast, Saccharomyces cerevisiae, during investigations of the chemical nature of the cytoplasmic genetic determinants concerned with the killer character in yeast.
Book ChapterDOI
[59] Resolution and isolation of yeast polysomes
Leon Marcus,Harlyn O. Halvorson +1 more
TL;DR: Polysomes are demonstrated in mammalian, bacterial, and yeast cells and procedure for the detection of polysomes and the isolation of individual size classes ofpolysomes in yeast is described.