Open Access
Xylanase production by Penicillium chrysogenum (PCL501) fermented on cellulosic wastes
TLDR
Out of the three agro-wastes used in this study, wheat bran holds the greatest promise for cost-effective production of the xylanase enzyme, as the carbon source is the highest inducer of the enzyme in the fungus.Abstract:
Xylanase production by Penicillium chrysogenum PCL501, newly isolated from wood-wastes, was
monitored at 24 h intervals for a period 168 h in media containing four different carbon sources (oatspelt
xylan, wheat bran, sawdust, and sugarcane pulp). The highest xylanase activity of 6.47 Units mL-1
was obtained at 96 h in media containing wheat bran whereas media containing sugarcane pulp gave a
peak value of 1.39 Units mL-1 at 144 h. Sawdust and xylan gave a peak xylanase activity of 1.35 and 0.79
Units mL-1 respectively at 120 h. Maximum protein released in xylan-containing media was 0.38 mg mL-1.
Higher protein yield was obtained in media containing the agro-wastes, with wheat bran giving the
highest value of 1.14 mg mL-1. The maximum specific xylanase activities were 2.59, 8.52, 16.06, and 9.36
Units mg Protein -1 for sawdust, sugarcane pulp, wheat bran and xylan respectively. Out of the three
agro-wastes used in this study, wheat bran holds the greatest promise for cost-effective production of
the xylanase enzyme. The carbon source is the highest inducer of the enzyme in the fungus.read more
Citations
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Potential of Penicillium Species in the Bioremediation Field
TL;DR: Several studies conducted with different strains of imperfecti fungi, Penicillium spp.
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Production, purification, and characterization of a major Penicillium glabrum xylanase using Brewer's spent grain as substrate.
Adriana Knob,Susan Michelz Beitel,Diana Fortkamp,César Rafael Fanchini Terrasan,Alex Fernando de Almeida +4 more
TL;DR: This study describes the xylanase production by Penicillium glabrum using brewer's spent grain as substrate, and is the first work that reports the purification and characterization of a xylan enzyme using this agroindustrial waste.
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One-factor-at-a-time (OFAT) optimization of xylanase production from Trichoderma viride-IR05 in solid-state fermentation
TL;DR: In this paper, the authors dealt with the production of enzyme xylanase by solid substrate fermentation using Trichoderma viride -IR05 and found that sugarcane bagasse was the best substrate for enzyme synthesis.
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Cost-Effective Production and Optimization of Alkaline Xylanase by Indigenous Bacillus mojavensis AG137 Fermented on Agricultural Waste
TL;DR: Produced xylanase was introduced as an alkaline-active and stable one, displaying suitable thermostability feature, confirmed by HPLC analysis, and all xylan enzyme properties highlight its promising uses in industrial scale.
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Xylanases: An Overview
TL;DR: Its distribution, structural aspects and industria l/ biotechnological applications are reviewed and studies related to cloning of the gene encoding endo-1,4-�≤ -xylanase with the objectives of overproducing the enzyme and altering its properties to suit commercial applications are discussed.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
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Multiplicity of beta-1,4-xylanase in microorganisms: functions and applications.
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Utilization of banana waste for the production of lignolytic and cellulolytic enzymes by solid substrate fermentation using two Pleurotus species (P. ostreatus and P. sajor-caju)
TL;DR: Leaf biomass was found to be a more suitable substrate compared to pseudostems for enzyme production and very low levels of cellulolytic enzyme activities were detected compared to lignin degrading enzymes by both the organisms.