Journal ArticleDOI
Yeast protoplasts immobilized in alginate: Cell wall regeneration and reversion to cells
Augustin Svoboda,Petr Ouředníček +1 more
TLDR
The method of protoplast immobilization and subsequent isolation from the gel is described in detail, and the morphology of the cell wall regeneration and morphology of reversion to the cell forms correspond to protoplasts development in gelatin or agar gels.Abstract:
Yeast protoplasts may regenerate the cell wall and revert to cells if immobilized in a 2%–5% Ca-alginate gel and cultured in an osmotically stabilized medium. The method of protoplast immobilization and subsequent isolation from the gel is described in detail. The reversion yield is dependent of the actual gel concentration, gel shape (beads vs. sheets) and of a medium molarity, and it may be up to 90%. The morphology of the cell wall regeneration and morphology of reversion to the cell forms correspond to protoplast development in gelatin or agar gels.read more
Citations
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Journal ArticleDOI
Gel entrapment and micro-encapsulation: methods, applications and engineering principles
Ronnie Willaert,Gino Baron +1 more
TL;DR: In this paper, the authors propose a method to solve the problem of "uniformity" and "uncertainty" in 3.5.5 GHz frequency bands, respectively.
Book ChapterDOI
Controlled Release of Immobilized Cells as a Strategy to Regulate Ecological Competence of Inocula
TL;DR: The ecological competence (the ability of microbial cells/inocula to compete and survive in nature) of laboratory/bioreactor prepared inocula is paramount to commercial exploitation of biotechnological processes initiated by the addition of microbial cultures to natural habitats.
Journal ArticleDOI
Microencapsulation: A Strategy for Formulation of Inoculum
TL;DR: A non-toxic phase separation method was developed for microencapsulation of inoculum used in biological control and mortality of Douglas-fir tussock moth larvae caused by a nuclear polyhedrosis virus was delayed when 23 polyhedral inclusion bodies were incorporated into alginate capsules.
Journal ArticleDOI
New materials and technology for cell immobilization
G.J. Salter,Douglas B. Kell +1 more
TL;DR: There is increasing interest in the use of tougher polymeric materials, and especially of inorganic ceramic supports, but the most suitable cell support can be selected only after the process or form of reactor in which it is to be used has been assessed.
Book ChapterDOI
Physiology of Immobilised Microbial Cells
TL;DR: A number of reports have appeared suggesting that the immobilisation has a profound effect on the metabolic behaviour of immobilised cells compared to free cells, but results of other reports suggest that the observed changes are caused by a change in local concentrations due to mass transfer limitations.
References
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Journal ArticleDOI
Diffusion characteristics of substrates in Ca-alginate gel beads.
TL;DR: The diffusion characteristics of several substrates of varying molecular sizes into and from Ca‐ alginate gel beads in well‐stirred solutions were investigated and the capacity of Ca‐alginate Gel as a matrix of immobilization was discussed.
Journal ArticleDOI
Molecular basis for some physical properties of alginates in the gel state
TL;DR: In this article, the authors show that the mechanical strength of calcium alginate gels is mainly due to junctions formed by the GG-blocks, and that the modulus of rigidity of gels formed by different cations is directly dependent on their ability to bind to polyuronides by a cooperative inter-chain binding mechanism.
Journal ArticleDOI
Regeneration, Cultivation and Differentiation of Plant Protoplasts Immobilized in Ca-alginate Beads
TL;DR: The major advantage of the method is improved cellular protection during the first critical weeks after isolation, which is a protection both against mechanical strain and strong gradients in environmental conditions.
Book ChapterDOI
Methods for the Immobilization of Microbial Cells
Joachim Klein,Fritz Wagner +1 more
TL;DR: This chapter discusses methods for the immobilization of microbial cells, which are those cells that are physically confined or localized in a certain defined region of space with retention of their catalytic activity and—if possible or even necessary—their viability.
Journal ArticleDOI
Immobilization of yeast cells by internal gelation of alginate
Anders Johansen,James M. Flink +1 more
TL;DR: The new gelation method exhibited promising characteristics with respect to fermentation rate and gel strength with the aim of immobilizing yeast cells in alginate particles by internal gelation.