How do you modify gene expression?
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|---|---|
| These now make it possible to generate targeted deletions, insertions, gene knock outs, and point variants; to modulate gene expression by targeting transcription factors or epigenetic machineries to DNA; or to target and modify RNA. | |
39 Citations | Furthermore, it provides a general frame on how to modulate and fine-tune gene expression of a target protein. |
| Modulating gene dose is an effective way to alter protein levels and modify phenotypes to understand gene function. | |
45 Citations | Gene targeting can be used to create point mutations, duplicate a gene, and modify the expression pattern of a protein almost as easily as creating knockout mice null for a particular protein. |
14 Citations | Developing a more "RNA centric" view of gene expression will allow a more systematic understanding of how gene expression and cell function are integrated. |
9 Citations | it would be possible to modify and study the expression of a gene in its natural chromosomal position in a whole animal. |
102 Citations | It is now possible to modify the expression of essentially any gene or partial gene product in the mouse heart at any time, either gain or loss of function. |
| These results indicate that the mPing insertion can be used as a genetic tool to modify an expression pattern of a target gene under stress conditions without changing the expression profiles of those under natural conditions. | |
| These findings reveal systematic, multilevel deregulation in gene expression and protein synthesis, showing how this major cellular process declines with age. | |
27 Citations | Alternatively, we attempted to modify plc expression through a promoter exchange approach but found the gene to be refractory to over-expression indicating that plc expression levels might additionally be tightly controlled. |
Related Questions
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What are the frequencies of mRNA modifications in specific RNA transcripts?5 answersRNA modifications, including N(6)-methyladenosine (m(6)A), 5-methylcytosine (m(5)C), pseudouridine (Ψ), and others, are proposed to fine-tune the structure and function of RNA. The frequencies of mRNA modifications in specific RNA transcripts have been studied. One study identified 231,836 specific RNA transcripts (SRTs) across various tissue and cancer types, most of which are found independent of specific genes. Another study focused on the location, regulation, and function of mRNA modifications, highlighting differences among modifications and between species. Additionally, the effects of environmental chemical exposure on mRNA modifications, such as m(6)A, have been investigated. Methods have been developed to accurately identify the position and modification fraction of mRNA modifications, allowing for functional investigation of RNA modifications. Overall, these studies provide insights into the frequencies and functional roles of mRNA modifications in specific RNA transcripts.
What is the newest modification in mRNA vaccine?5 answersThe newest modification in mRNA vaccines involves optimizing the structural elements of the mRNA molecules. These modifications include synthesizing an advanced cap structure, incorporating untranslated regions (UTRs), and extending the poly(A) tail. These modifications aim to improve the expression level of encoded proteins and induce potent immune responses with a relatively low dosage. Additionally, modifying the 5' cap, 5'-and 3'-untranslated regions (UTRs), the coding region, and polyadenylation tail helps reduce excessive mRNA immunogenicity and improve intracellular stability and translational efficiency. The success of COVID-19 vaccines has also been enabled by previous innovations in nucleoside modifications during in vitro transcription. Ongoing developments in mRNA immunotherapy include modifications in the route of administration and combined delivery of multiple mRNA vaccines with checkpoint inhibitors.
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