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The assay is quick, convenient, and in principle measures functionally available zinc.
It is thought that zinc may play a part in this process.
Zinc status of the individual may also influence adjustments in fractional zinc absorption.
This process is probably independent of the zinc concentration.
Zinc absorption in humans could be improved by zinc complexation with gluconate.
It is also hypothesized that the differences between TM and zinc may be caused by TM's mechanism of action in which it binds copper already in the body, whereas zinc does not.
The model is able to explain the experimental evidence collected so far in our laboratory: high zinc yields are only obtained if the zinc/oxygen mixture is strongly diluted with inert gas.
However, the effect of zinc in reducing the extraction of copper is significant.