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How to separate ortho-salicylic acid and para-salicylic acid by LC? 

Chiral column chromatography
Chiral derivatizing agent
Atmospheric-pressure chemical ionization
Chiral resolution
Trifluoroacetic anhydride
Best insight from top research papers

Ortho-salicylic acid and para-salicylic acid can be separated by liquid chromatography (LC) using different methods. One approach is to use a chiral column, such as an immobilized amylose-based Chiralpak-IA column, with a mobile phase consisting of n-hexane/ethanol/trifluoroacetic acid/diethyl amine (65:35:0.1:0.1, v/v) . Another method involves using a monochromatic laser beam to align atoms into a single nuclear spin state, which has been successful in achieving ortho-para separations of certain molecules . Additionally, high-performance liquid chromatography on macrocyclic glycopeptide based chiral stationary phases can be used to enantioseparate certain compounds, including γ-amino acids . Furthermore, chiral stationary phases synthesized by covalent bonding of aminopropylsilica to specific compounds have been found suitable for the chiral resolution of certain dinitrophenyl and dinitrobenzoyl-DL-amino acid esters . Therefore, there are multiple methods available for separating ortho-salicylic acid and para-salicylic acid by LC, depending on the specific compounds and conditions.

Answers from top 5 papers

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Papers (5)Insight
Journal Article•DOI
Simultaneous extraction of acetylsalicylic acid and salicylic acid from human plasma and simultaneous estimation by liquid chromatography and atmospheric pressure chemical ionization/tandem mass spectrometry detection. Application to a pharmacokinetic study.
Ramakrishna Nirogi, Vishwottam Kandikere, Koteshwara Mudigonda, Devender Reddy Ajjala, Ramakrishna Suraneni, Parthasarathi Thoddi 
01 Jan 2011-Drug Research
13 Citations
The provided paper does not provide information on how to separate ortho-salicylic acid and para-salicylic acid by LC. The paper focuses on the simultaneous extraction and estimation of acetylsalicylic acid and salicylic acid from human plasma using liquid chromatography-tandem mass spectrometry.
Journal Article•DOI
LC-separation of derivatized DL-amino acids by aminopropylsilica-bonded Marfey's reagent and analog
Hans Brückner, Hans Brückner, M. Leitenberger 
01 Jun 1996-Chromatographia
12 Citations
The provided paper does not mention the separation of ortho-salicylic acid and para-salicylic acid by LC.
Journal Article•DOI
LC Separation of para and meta Isomers of Zafirlukast in Bulk Drug Samples and Pharmaceutical Dosage Forms Using a Chiral Stationary Phase
A. Madhavi, D. V. Subba Rao, Andra Naidu, P. Raghuram 
28 Jun 2009-Chromatographia
1 Citations
The provided paper does not mention anything about the separation of ortho-salicylic acid and para-salicylic acid by LC.
Journal Article•DOI
Ortho-para separation of molecules using optical orientation of atoms in hyperfine Zeeman states
Chun He
01 May 1998-Physical Review A
2 Citations
The provided paper does not discuss the separation of ortho-salicylic acid and para-salicylic acid by LC.
Journal Article•DOI
LC Separation of γ-Amino Acid Enantiomers
Zoltán Pataj, István Ilisz, Anita Aranyi, Enikő Forró, Ferenc Fülöp, Daniel W. Armstrong, Antal Péter 
05 Feb 2010-Chromatographia
10 Citations
The provided paper does not mention the separation of ortho-salicylic acid and para-salicylic acid by LC.

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