How does the presence of chitosan affect the process of in situ precipitation of calcium phosphate?5 answersThe presence of chitosan significantly influences the in situ precipitation of calcium phosphate. Chitosan plays a crucial role in directing the growth and assembly of calcium phosphate crystals, leading to the formation of unique structures like flower-like microparticles. Additionally, chitosan, when combined with simulated body fluid (SBF), promotes the formation of hydroxyapatite, a key component of bone tissue, providing insights into biomineralization mechanisms. Chitosan's impact extends to the realm of nanocomposite materials, where its addition to silicate cross-linked poly(ethylene oxide) enhances cellular adhesion, bioactivity, and biomineralization control, making it a valuable component for creating bioactive scaffolds for bone repair. Overall, chitosan's presence influences the morphology, structure, and properties of calcium phosphate-based materials, showcasing its versatility in biomineralization processes.
How does the presence of chitosan affect the formation of calcium phosphate crystals?5 answersThe presence of chitosan influences the formation of calcium phosphate crystals by affecting their physico-chemical properties and adherence strength to substrates. Chitosan incorporation into calcium phosphate layers results in the formation of a calcium-deficient hydroxyapatite structure when deposited at room temperature. Chitosan-coated calcium phosphate films exhibit oriented crystals and qualitative control of calcium phosphate phases, promoting the growth and nucleation of porous calcium phosphate coatings. Additionally, chitosan accelerates the hardening dynamics of calcium phosphate cement, reducing the amount of unreacted monomers and enhancing the hardening process. Chitosan, along with chitin, chelates calcium and phosphate ions, providing an ambiance for hydroxyapatite crystallite formation, leading to the formation of micro-crystallites with specific calcium to phosphate ratios. Overall, the presence of chitosan plays a crucial role in modifying the structure and properties of calcium phosphate crystals.
How does the presence of chitosan affect the growth rate of calcium phosphate crystals?5 answersThe presence of chitosan influences the growth rate of calcium phosphate crystals in various ways. Chitosan incorporation into calcium phosphate layers can lead to the formation of a calcium-deficient hydroxyapatite structure, affecting the physico-chemical properties. Additionally, chitosan accelerates the hardening dynamics of calcium phosphate cement, reducing the amount of unreacted monomers within the cement over time. Furthermore, chitosan-coated chitosan films with porous calcium phosphate coatings have shown the ability to induce the growth and nucleation of calcium phosphate crystals, mimicking natural tissue formation. Chitosan's presence in hydroxyapatite 3D scaffolds/composites does not significantly alter the Young's modulus or antimicrobial properties, making them suitable for biomaterial applications. The chitin-chitosan-hydroxyapatite system provides an ambiance for HA crystallite formation, resulting in a bioactive surface with good biocompatibility and a structure resembling native bone.
What are the current methods used for testing the in vitro biodegradability of CHITOSAN-BASED BIOACTIVE COATING?5 answersThe current methods for testing the in vitro biodegradability of chitosan-based bioactive coatings include assessing the cytotoxicity of Chitosan-Cu complexes on surrounding cells through the CCK-8 test kit, as demonstrated in a study by Erişen et al.. Additionally, the biodegradation control of chitosan materials can be achieved by using random copolymers based on glycidyl methacrylate and (fluoro)alkyl methacrylates as surface modifiers, as reported by Yus. Furthermore, the incorporation of poly(ethylene glycol) as a sacrificial additive in electrospinning can lead to the preparation of fully biodegradable chitosan/TMC nanofibers, enabling high swelling ability, controlled biodegradability, and in vitro and in vivo biocompatibility, as discussed by an article by an undisclosed author.
What factor that influence the characteristic of chitosan?5 answersThe characteristics of chitosan are influenced by various factors such as its molecular weight, concentration of acetic acid, chitosan flakes, and tripolyphosphate (TPP). Different molecular weights of chitosan affect its properties, with low molecular weight chitosan showing better transfection efficiency in gene therapy applications. Additionally, the molecular weight of chitosan impacts the color properties and rheological behavior of chitosan and gels when exposed to gamma irradiation. Moreover, the antibacterial activity and physical properties of chitosan membranes are influenced by the molecular weight of chitosan, with high-molecular-weight chitosan showing the best antibacterial activity against certain bacteria strains. These factors collectively play a crucial role in determining the overall characteristics and performance of chitosan in various applications.
What are the chitosan materials that can be used for corneal replacement?4 answersChitosan materials that can be used for corneal replacement include soluble chitosan crosslinked with gelatin and chondroitin sulfate to obtain blend membranes. Another option is the use of decellularized corneal matrix from pigs combined with chitosan to create a composite material. Additionally, chitosan-based green composites, such as chitosan nanocomposites and chitosan blends with natural fibers, have potential applications in corneal tissue engineering. Furthermore, corneal matrix cells can be co-cultured with chitosan scaffolds to construct tissue-engineered corneal matrices. These studies demonstrate the versatility of chitosan in corneal replacement materials and highlight its potential for use in corneal tissue engineering and biomedicine.