What amino acids do azido groups crosslink with?
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Azido groups can crosslink with various amino acids such as serine, alanine, lysine, and tyrosine, forming novel bis-amino acids using click chemistry . Additionally, modified amino acids containing azido groups are utilized in the synthesis of polypeptides, antibodies, and conjugates for various applications in treatment, prevention, detection, and diagnosis . The use of azido acids as protecting groups in peptide synthesis is highlighted due to their atom efficiency, ease of installation, and compatibility with other protecting groups, making them ideal for the synthesis of branched and cyclic peptides . Furthermore, the mapping of cross-links in proteins is facilitated by cleavable cross-linkers like bis(succinimidyl)-3-azidomethyl glutarate (BAMG), which provide valuable distance constraints for validating protein 3D structures .
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47 Citations | Azido groups in the cross-linker BAMG primarily crosslink with lysine residues in proteins, facilitating mapping of lysines in close proximity for structural analysis. |
| Azido groups in modified amino acids can crosslink with other functional groups like alkynes or strained cyclooctynes, enabling bioconjugation for various applications in treatment, prevention, detection, and diagnosis. | |
| Azido groups in modified amino acids can crosslink with other functional groups such as alkynes or strained cyclooctynes, enabling bioconjugation for various applications in biotechnology and medicine. | |
1 Citations | Azido groups crosslink with α-amino acids to form azido acids, which are crucial for synthesizing complex peptides due to their atom-efficient and versatile properties. |
4 Citations | Azido groups crosslink with serine, alanine, lysine, and tyrosine amino acids in the synthesis of crosslinking bis-amino acids using click chemistry as described in the paper. |
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