What are the reasons behind protein loss expressed in E.coli during fermentation process?
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Protein loss in E. coli during fermentation can be attributed to various factors. Firstly, the drop in viable cell count post-induction may reflect a loss of cell division capability rather than cell death, impacting protein production. Additionally, the appearance of plasmid-losing cells during cell fission can lead to protein loss, emphasizing the need for strategies to maintain plasmid stability. Moreover, overproduction of NADPH in the pentose phosphate pathway can repress cell growth, affecting protein expression levels. Furthermore, high-level over-expression of both soluble and integral membrane proteins can result in significant cell loss, impacting protein yields. Understanding these factors is crucial for optimizing recombinant protein expression in E. coli fermentation processes.
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| Papers (5) | Insight |
|---|---|
| High-level over-expression of both soluble and integral membrane proteins in E. coli triggers >99.9% cell loss, leading to an adaptive survival response akin to antimicrobial resistance development. | |
| Protein loss in E. coli during fermentation is due to variations in gene expression, such as ptsG degradation, affecting glucose consumption and biomass concentration, as observed in pgi mutants. | |
| Protein loss in E. coli during fermentation is due to plasmid instability caused by cell fission generating plasmid-losing cells, leading to competitive advantage and rapid population dominance. | |
25 Citations | Metabolic burden, inclusion bodies, and inefficient translocation contribute to protein loss during E. coli fermentation, emphasizing the need for optimizing transcription to enhance recombinant protein production efficiency. |
28 Citations | Protein loss in E. coli during fermentation is mainly due to a drop in cell division capability, not cell death, as observed in recombinant protein production processes. |
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