Kazuyuki Shimizu

TL;DR: E. coli seems to use complementary strategies that result in a metabolic network robust against perturbations, and actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate.

TL;DR: By evaluating the energy economy through the energy utilization efficiency, it was found that the biomass yield on the supplied energy was the lowest in the autotrophic cultivation, and the cyclic culture gave the most efficient utilization of energy for biomass production.

TL;DR: The analyzed result suggested that the activity of the respiratory electron transport chain in cyanobacterial cells was not inhibited by light, and revealed that the NADPH synthesis was in excess relative to its requirement, and the excess NADPH might be reoxidized in Cyanobacterial respiration to provide the energy for cellular requirement.

TL;DR: Regulation of the main metabolic pathways of Escherichia coli K12 was investigated based on 2-dimensional electrophoresis (2DE) and the measurement of enzyme activities, and protein abundance obtained by 2DE correlated well with enzyme activity, with a few exceptions.

TL;DR: Although knockout of G6P dehydrogenase had no significant influence on the central metabolism under glucose-limited conditions, this mutation resulted in extensive overflow metabolism and extremely low tricarboxylic acid cycle fluxes under ammonia limitation conditions.