What is benedict solution?
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Benedict solution is a reagent used to detect reducing sugars, particularly glucose, in various applications such as urine tests for diabetes detection . It consists of a mixture of copper sulfate and sodium citrate, which reacts with reducing sugars to form a colored precipitate. Benedict solution is commonly employed in place of Fehling's solution for its effectiveness in identifying excess sugar in urine, indicating potential diabetes . The solution's color change during the reaction helps determine the presence and concentration of reducing sugars, making it a valuable tool in qualitative tests for glucose . Additionally, the Benedict reaction has been enhanced using 2,2′-bicinchoninic acid to increase sensitivity, enabling a more precise detection of copper(I) in the test .
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| Papers (5) | Insight |
|---|---|
| Benedict solution is a test reagent used to detect easily oxidizable compounds by producing copper(I) oxide precipitates. The paper introduces a more sensitive Benedict test using 2,2'-bicinchoninic acid. | |
| Not addressed in the paper. | |
6 Citations | Benedict's solution is a qualitative test for urine sugar, established in 1909 for diabetes detection. It is compared with Clinitest, Tes-Tape, and Clinistix in the research study. |
2 Citations | Benedict's solution is a developer used in carbohydrate chromatography to detect reducing sugars, addressing challenges of background staining by other reagents like silver nitrate. |
| Benedict solution is used in glucose testing, replacing Fehling solution. It changes color when heated with urine; green indicates low sugar, red precipitate indicates high sugar levels. |
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How do you prepare a solution?5 answersA solution can be prepared by following a specific method. First, the active ingredients are dissolved in a solvent to obtain a solution A. Then, an osmotic pressure regulator and a pH regulator are added to solution A to obtain a solution B. Solution B is then filtered and encapsulated in an inner packaging layer made of a semi-permeable material to obtain the finished product. The finished product is filled in an outer packaging layer, which is sealed and filled with inert gas or nitrogen to prevent contamination. This method eliminates the need for additional nitrogen equipment and reduces equipment requirements and cost. Another method involves calculating the target liquid level height for each container, pouring the raw materials into the first container until the liquid level reaches the target height, and obtaining the mixed solution with precise control over the quantity of each raw material. Additionally, a method and device allow for automatic preparation of a solution by controlling a substance adding device based on the preparation information. Another method involves adjusting the concentration of a first solution until it falls within a specified range, allowing it to stand, and then analyzing and determining its concentration before splitting it into finished products. Finally, a method for preparing a developing solution involves dissolving salicylate in benzyl alcohol, adding a main developing agent, and sequentially adding other ingredients to obtain the final solution.
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Advantages and disadvantages of benedict's method instead fehlings's method for detecting urinary reducing agents like glucose?3 answersBenedict's method has advantages and disadvantages compared to Fehling's method for detecting urinary reducing agents like glucose. One advantage of Benedict's method is that it can detect a wide range of reducing agents, including glucose, and provide qualitative results from negative to positive. This is shown in the study by Fitri Fadhilah and Noviana Vanawati. However, there are some disadvantages to using Benedict's method. The process of urine reduction examination using a flame requires a longer time and can be time-consuming, as it can only be done one sample at a time. There is also a risk of the solution in the tube exploding. On the other hand, Fehling's method, although not mentioned in the provided abstracts, is known to be more specific for glucose detection and can provide quantitative results. It is also less time-consuming and can be done with multiple samples simultaneously.