What results were obtained studying antibody aggregation ?
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Antibody aggregation was studied in several papers. One study found that high concentration antibody solutions with rich multimers had a deeper color, indicating the presence of multimers. LC-MS analysis showed that the multimers had a complex structure and ICP-MS analysis revealed a higher concentration of iron ions in the multimers . Another study used in silico analysis to investigate antibody aggregation and found that the presence of glycans in the Fc region of antibodies influenced the aggregation prone regions (APR) of the antibodies . A third study investigated the effect of hydrodynamic flow and solid-liquid interfaces on protein aggregation and found that changes in the materials of the syringe barrels had a dramatic effect on protein aggregation, while increasing shear rate had a negligible effect . A fourth study used numerical simulations to study the hydrodynamic behavior of antibodies near silicone oil-water interfaces and found that protein loss from the bulk solution occurred as the molecules adsorbed at the interface . Finally, a study investigated the interaction of immunoglobulin G (IgG) antibodies with gold nanoparticles (AuNPs) and found that the addition of antibody triggered the aggregation of AuNPs at pH < 7.5, while a stable bioconjugate was formed at pH ≥ 7.5 .
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16 Citations | The results obtained from studying antibody aggregation showed that the addition of antibody triggered the aggregation of gold nanoparticles (AuNPs) at pH < 7.5, but a stable bioconjugate was formed at pH ≥ 7.5. The mechanism of aggregation was identified as electrostatic bridging between the antibody and the negatively charged AuNPs. The protein-AuNP interactions were reversible within the first few hours but became irreversible after 24 hours. |
32 Citations | The study found that increasing shear rate had a negligible effect on protein aggregation, while changes in the materials of the syringe barrels had a dramatic effect on monomer loss. |
| The results obtained from studying antibody aggregation showed that the presence of glycans in the Fc region of antibodies can influence and potentially protect hydrophobic regions associated with aggregation. However, the size of the glycans may limit their influence on aggregation. | |
1 Citations | The results obtained from studying antibody aggregation include the observation of a deeper color in high concentration antibody solutions with rich multimers, as well as the identification of iron ions as an important factor in the formation of multimers. |
6 Citations | The paper discusses the results of studying antibody aggregation near silicone oil-water interfaces, including protein loss from the bulk solution, dynamic cluster formation, and the effect of antibody concentration on diffusivity and aggregation propensity. |
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