Showing papers on "Color reaction published in 1980"

Determination of Glucose by a Modification of Somogyi-Nelson Method

Abstract: Nelson’s arsenomolybdate, the chromogenic reagent in Somogyi–Nelson method, was replaced by Folin–Ciocalteu phenol reagent. The major object was to remove the toxic arsenic compounds from the color reaction system. The color-producing ability of the phenol reagent was considerably lower than that of Nelson’s reagent. However, the modified method was favorably comparable to Somogyi–Nelson method in simplicity, reproducibility and stability of color development. The error in both the modified and Somogyi–Nelson method could be reduced to about one fourth by adding sodium benzoate (final concentration, about 0.5%) to the test solutions.

Automated colorimetric determination of acid proteinase activity in fermentation samples using a trinitrobenzenesulphonic acid reagent

Abstract: An automated method for the determination of acid proteinase activity has been developed. The enzyme is incubated with a haemoglobin substrate after which the reaction solution is dialysed. The concentration of the liberated amino acids and peptides is determined by reaction with a trinitrobenzenesulphonic acid reagent. The technical parameters of the process and the general analytical conditions have been optimised. The method offers great advantages in its flexibility of selecting substrate and buffer independently of the conditions for the colour reaction. The final result is an automated method with high sensitivity, precision and speed. The sensitivity of the colour reaction is 1 mg l–1 of nitrogen from leucine.

Test tube construction and method for measuring for nickel aerosols

Abstract: A testing device for measuring for nickel-aerosols includes a glass tube having breakable end tips for opening the tube for the passage of a test gas therethrough and a filling in the tube which includes a closed ampoule of hydrochloric acid, a filter layer for entrapping nickel particles of the gas being tested, a reaction layer of a material impregnated with a color reagent for nickel, a granular buffer layer and a second breakable ampoule of ammonia water arranged in the direction of flow of the test gas through the tube. The testing is carried out by breaking the first ampoule to release hydrochloric acid which flows through the filter and reacts with the nickel particles which pass to the reagent layer, subsequently breaking the second ampoule so that its contents will be directed into the granular buffer layer and in the reagent layer. The ammonia water in the second ampoule is directed into the buffer layer and then into the reagent layer to produce a color reaction in the reagent layer and the intensity of the discoloration produced is proportional to the amount of nickel filtered out of the test gas. This color is compared with a standard showing the color of reaction for various concentrations of nickel.

Synthesis of 4, 5, 6, 7-Tetrahydro-1, 1-dimethyl-2-(4'-methyl-penta-1', 3'-dien-1'-yl)-indene. A Synthetic Proof of the Chromophoric Structure of a Colour-producing Compound in the Colour Reaction of Retinoic Acid in 74% Sulphuric Acid

Abstract: The title compound (3) which involves a new conjugated system, has been synthesised in a geometrically pure form the indanone (4).

Identification of 1,5-anhydroglucitol in thin-layer chromatograms.

Abstract: The chromatographical characteristics of 1,5-anhydroglucitol in thin-layer chromatograms can be studied using the customary carbohydrate solvents. Both hydrogen peroxide and sodium metaperiodate oxidation reactions were tested in order to find a specific colour reaction for the detection of the compound. 1,5-anhydroglucitol was readily converted by periodate into an intermediate product which produced an intense orange-red colour with diphenylamine aniline reagent. According to data obtained from periodate oxidation, IR spectroscopy and mass fragmentography, the intermediate product was a dialdehyde compound with a C6 structure, possibly formed through cleavage at C2-C3. The formation of a compound of this kind without chain cleavage in the periodate oxidation of C6 carbohydrates is uncommon. Periodate oxidation followed by diphenylamine-aniline reaction affords a sensitive and specific method for the detection of 1,5-anhydroglucitol.

Selective colorimetric detection of carboxylic acids

Abstract: A selective colour reaction for the detection of carboxylic acids has been developed. Acetic anhydride in the presence of ammonium carbonate, sodium carbonate and the sodium salts of acetic, formic, oxalic and pyruvic acids is used as the reagent. Amongst 39 acids, citric, isocitric, α-ketoglutaric and oxaloacetic acids can be detected selectively.

Living thing active material from plant of composite artemisia and its preparation

Abstract: NEW MATERIAL:An interferon inducing material being stable in the condition of amorphous white powder. Elemental analysis, C 45.6+ or -0.4%, H 7.4+ or -0.4%, N 13.5+ or -0.4%, P 2.8+ or -0.3%. Molecular weight, about 100,000-3,000,000 (mainly about 500,000-1,000,000). Specific rotary power, [alpha] D=+73 deg.-79 deg. (C=0.47, 0.1N NaOH). Color reaction, positive in ninhydrin reaction and Dittmer reaction and negative in Folin's reagent and Elson-Morgan's reaction. USE:An interferon inducing material. Useful for prevention and curing of a disease infected by virus, e.g., carcinogenic virus. PROCESS:The active material is extracted from stalks and leaves of a plant of Composital Artemisia (e.g., A. princeps Pampanini, A. Capillasis Thunberg, etc.) with an alkali aqueous solution. The extracted solution is purified by ultrafiltration, etc. to give the desired interferon inducing material.

Colour reaction of dichlorphenamide and related sulphonamide drugs

Abstract: A specific colour reaction for the determination of dichlorphenamide is reported. The method is based on the reaction of dichlorphenamide with an aliphatic amine,n-butylamine, and cobaltous chloride in anhydrous methanol, a blue-violet colour is produced and is measured at the wavelength of maximum absorbance at 570 nm. The developed colour is suggested to be specific for the aromatic unsubstituted sulphonamide groups, the specificity of the reaction is elucidated through application to other related sulphonamide compounds.

Multilayer material for chemical analysis

Abstract: PURPOSE: To analyze quantitatively components in a living body fluid, by using a multilayer chemical analyzing material comprising a colorless substrate layer having a pigment forming reactive group and containing a nondispersive substrate, and a color reaction layer containing a chromogen compound. CONSTITUTION: From a color reaction layer containing (A) a substrate layer having a colorless pigment-forming reactive group [examples; 4,6-bis(8-hydroxy-3,6- disulfo-1-naphthylamino)-s-triazinc-2-il group and so on] able to form a pigment by reacting with a chromogen compound described in the following, and containing a nondispersive substrate able to produce said colorless diffusive compound having the pigment forming reactive group by the action of the object to be inspected, and (B) a chromogen compound (for examples; 2-tetradecyloxy-5-sulfonate benzenediazonium, etc.,) able to form a pigment by reacting with said pigment forming reactive group on an optical transmissive (for example; a plate such as polyetylene telephthalate), a material mentioned in the face that analyzes quantitatively components in a living body fluid. COPYRIGHT: (C)1982,JPO&Japio

Novel bioactive substance

Abstract: NEW MATERIAL:A bioactive substance P-1894 B having the following characteristics: melting point, 165-172 deg.C; elemental analysis, C 62.62+ or -1.0, H 6.28+ or -0.5, O 30.06+ or -1.0; molecular weight, about 1,000; specific rotation, [alpha]D =155+ or -15 deg. (C=0.419, in methanol); color reaction: naphthoresorcin-sulfuric acid reaction, positive; solubility, soluble-easily soluble in chloroform, dioxane, etc., slightly soluble-insoluble in water, 0.1N HCl, petroleum ether, etc.; weakly acidic substance; orange yellow-red yellow crystal. USE:The substance having protocollagen.proline-hydroxylase-inhibiting activity and collagen-biosynthesis-suppressing activity. PROCESS:Streptomyces sp. No.1894 (IFO 13881) is cultured in a liquid medium at 20-40 deg.C and pH4-9, and the bioactive substance P-1894 B is separated from the medium by adsorption chromatography, etc.

Weakly basic oligosaccharide amylostatin and its preparation

Abstract: NEW MATERIAL:Wealky basic oligosaccharide amylostatins J, K, L, M and N. Having values of elementary analysis different from each other amoung physical and chemical properties of amylostatins. Molecular weights as shown by the table. What are common to all; melting point: gradually are carbonized but not show clearly; having no specific absorption maximum at ultraviolet part; solubility: easily soluble in water, pyridine, etc., slightly soluble in ethanol, methanol, and insoluble in acetone, ether, etc.; color: white powder; color reaction: anthrone sulfuric acid reaction positive, ninhydrin reaction negative. USE:Having inhibiting actions on glycoside hydrolysis enzyme and immunity enhancing actions. PROCESS:A bacterium (e.g., ''Streptomyces diastaticus var. amylostaticus'' FERM-P 2499) belonging to the genus ''Streptomyces'' and having amylostatin producing ability is cultivated in a nutrient medium. The titled amylostains J-N are obtained from the culture solution.

Novel antibiotic ah2589

Abstract: NEW MATERIAL:AH2589-I having the following properties. Molecular weight: 2000-10,000 (gel filtration method). Easily soluble in water, soluble in water- containing methanol, water-containing acetone, etc., slightly soluble or insoluble in methanol, acetone, chloroform, benzene. Color reaction, positive in Rydon and Smith, copper-Folin's reaction, and negative in ninhydrin reaction. The amino acid analysis after its hydrolysis by hydrochloric acid has shown that it contains lysine, histidine, arginine, glycine, alanine, valine, (iso)leucine, phenylalanine. USE:An antibacterial agent for gram-negative and gram-positive bacteria. PROCESS:For example, streptomycetaceae globisporus AH (FERM-P No.4706) is cultured with aeration under stirring at 25-30 deg.C for 40-150hrs, separated and purified by column chromatography.

Color Reaction Product of Urea with Diacetyl Monoxime and Glucuronolactone. III. Structures of Two Major Reaction Products of the Color Reaction of Butylurea with 1-Phenyl-1, 2-propanedione

Abstract: Two crystalline color reaction products were isolated from the reaction mixture of butylurea with 1-phenyl-1, 2-propanedione in dioxane containing HCl. One of them (A) was found to be identical with 1, 3'-dibutyl-5, 5'-diphenyl-2, 2'-dioxo-4, 4'-diimidazolylmethane (I) which was isolated previously. The other one (C) was found to be isomeric with compound (B) which was also isolated previously. It was found that the only difference between B and C is the positions of their butyl side chains. In order to decide the positions of the side chains of B and C, the differences between the physico-chemical properties expected to be shown by 3, 3'-dibutyl-(II) and by 1, 1'-dibutyl-5, 5'-diphenyl-2, 2'-dioxo-4, 4'-diimidazolylmethane (III) were estimated theoretically. From the results of this estimation the structures II and III could be assigned to B and C, respectively.