Showing papers on "Plasmid recombination published in 1982"
TL;DR: Recombination-induced oligomer formation from monomeric plasmids was found to be dependent on the functions of the recA, recB, recC and recF genes, and a role for the recB recC gene product, exonuclease V, in the resolution of recombination intermediates is discussed.
114 citations
TL;DR: The complete nucleotide sequence of trpS, the structural gene for Escherichia coli tryptophanyl-tRNA synthetase, was determined using a plasmid carrying the structural genes using currently available procedures to predict the secondary structure for the enzyme from each organism and compared these structures to those of the two aminoacyl-t RNA synthetases whose three-dimensional structures have been determined.
66 citations
TL;DR: Genetic analysis led to the conclusion that each of the two mutants carried a mutation in the k1 plasmid and that the recovery of killer phenotype was due to genetic recombination of the plasmids, and not to complementation.
Abstract: The killer character of Kluyveromyces lactis is cytoplasmically inherited. Two DNA plasmids k1 and k2 (8.8 and 13.4 kilobase pairs, respectively) are associated with the killer system. Either cytoplasmic or nuclear mutations can lead to a loss of the killer activity. Two cytoplasmic non-killer mutants have been specifically studied. When they were crossed to each other, the killer phenotype was restored. Genetic analysis led us to conclude that each of the two mutants carried a mutation in the k1 plasmid and that the recovery of killer phenotype was due to genetic recombination of the plasmids, and not to complementation.
47 citations
TL;DR: DNA recombination in exponential phase and competent Haemophilus influenzae was measured by an electron microscopic assay that relies on the conversion of plasmid RSF0885 monomers into multimeric forms and revealed the presence of recombination intermediates composed of two linear plasmids joined to form two pairs of arms resembling the Greek letter chi.
13 citations
01 Nov 1982
TL;DR: The influence of ethidium bromide, actinomycin D and acridine orange treatment of Bacillus subtilis competent cells on plasmid transformation, recombination and transduction was studied and it was probably the result of damaging the synaptic stage of recombination because of DNA conformational changes.
Abstract: The influence of ethidium bromide, actinomycin D and acridine orange treatment of Bacillus subtilis competent cells on plasmid transformation, recombination and transduction was studied. The drugs were administered at different times after DNA uptake. The yield of plasmid recombinants and transformants was inhibited in different ways during the first 30 minutes. Probably, it was the result of damaging the synaptic stage of recombination because of DNA conformational changes. The effect of drugs on plasmid transduction was significantly lesser.
2 citations