Showing papers on "Semen analysis published in 1979"

Improvement of Semen and Pregnancy Rate after Ligation and Division of the Internal Spermatic Vein: Fact or Fiction?

Abstract: Male partners with left-sided varicoceles of 96 infertile couples were studied. 51 patients were submitted to ligation of the testicular veins and 45 individuals were randomized as controls. During an observation period of 53 months (range of 36-74 months) we found no statistically significant improvement in the semen crude variables the morphology or progressive motility in the series of men who underwent surgery. The pregnancy rate was lower in those who had their varicoceles excised. (authors modified)

Within-subject variability of human semen in regard to sperm count, volume, total number of spermatozoa and length of abstinence

Abstract: The within-subject variability of the semen sperm count (n), volume (v) and the total number of spermatozoa (N) was studied on 220 ejaculates from 36 normal subjects after an abstinence of 7 days or less. For each of the three variables, the within-subject standard deviation, sigma, was practically proportional to the mean, mu; the common value of the coefficient of variation sigma/mu for all subjects was very high: 0.39 for n, 0.28 for v and 0.55 for N. The 95% confidence intervals based on a single ejaculate were asymmetrical and very large, the lower and upper limits being respectively 0.5 x n and 2.3 x n; 0.7 x v and 1.8 x v; 0.4 x N and 2.9 x N. The three semen characteristics for a given subject were highly correlated with length of abstinence: for an increase in abstinence of 1 day there were mean increments of 13 x 10(6)/ml for n, 0.4 ml for v, and 87 x 10(6) for N.

Index of Longevity — A new Definition of an Index for Sperm Quality Evaluation

Abstract: A new index by which sperm longevity can be evaluated quantitatively is suggested for a routine semen analysis, and was calculated in seminal specimens of 50 donors. This index of longevity is defined according to each one of 2 different characteristics of sperm motility analyzed by the MEP method: ILM — the ratio between per cent of motile sperm measured four h and one h after ejaculation. ILV — the ratio between sperm velocity measured four and one h after ejaculation. The ratio clearly shows whether motility, tested in vitro, is stable or drops rapidly with time. ILM was found to range between 0.2 and 1.3 with average 0.8. ILV ranged from 0.5 to 1.8 and average 1.14. Neither ILM nor ILV showed any correlation with sperm concentration, abnormal forms, initial per cent of motility or initial sperm velocity and thus it was impossible to predict sperm longevity from any known sperm characteristics. However. a coefficient of correlation of 0.44 was found between ILM and ILV which means that to a certain extent, cases with well-preserved percentage of motility may also reveal a well-preserved sperm velocity. With the aid of this index it is possible to investigate if there is any correlation between sperm longevity and fertility rate, sperm survival in various media, alleged sperm stimulants or treatment given to the male.

Some biochemical parameters for qualification of bull semen.

Abstract: Since the macroscopical and microscopical examination of bull semen does not in all cases appear to give conclusive indications concerning the fertilizing capacity, an investigation of some biochemical parameters was undertaken. It was shown that the following biochemical examination could furnish some more information about the quality of the semen: (1) Determination of the phenylalanine- alpha-ketoglutarate transminase activity both in the seminal plasma and in the whole semen. A high transminase activity in the plasma points to leakage out of the spermatozoa, thus indicating an affection of the spermatozoa. In that case larger amounts of basic amino acids and of leucine were often found in the seminal plasma than would normally be observed. (2) Gas chromatographic examination of the steroids occurring both in the seminal plasma and in the spermatozoa. In a number of infertile bulls small peaks of progesterone were found together with those of other, unidentified compounds. (3) Investigation of the carbohydrate metabolism in the semen. In a number of fertile bulls the following deviations were found: a. the presence of quite a lot of fructose in the spermatozoa; b. the presence of glucuronic acid and/of other foreign compounds in the spermatozoa or in the plasma; c. an elevated pH in the seminal plasma some 2 to 3 hours after ejaculation; d. a low phenylalanine- alpha-ketoglutarate transaminase activity in the whole semen (the spermatozoa had been disrupted by freezing). (4) Investigation of the presence of reducing aldehydes (glycoladehyde and glyceraldehyde) inside the spermatozoa. The determinations mentioned under 1, 3c and 3d can easily be carried out in the laboratories of each District Animal Health Service and of the A.I. stations; the same is true of the occurrence of amino acids in the seminal plasma and of fructose and aldehydes inside the spermatozoa if facilities are available for electrophoresis and chromatography.

Local male hormonal therapy in male infertility: a preliminary report.

Abstract: The therapeutic effect of local infiltration of the testis by testosterone crystalline suspension was studied in 18 cases of severe oligozoospermia. Evaluation was done by semen analysis follow-up, and results were correlated with preoperative testicular biopsies. About 40% of the cases showed improvement of sperm count and/or motility. Testicular biopsies of responding cases showed mild inhibitory changes, such as sloughing or partial spermatogenic arrest at late stages, while cases with tubular hyalinization or spermatogenic arrest at the stage of spermatogonia showed no response. The method proved to be simple, safe, and promising.