Adam P. Hall

TL;DR: This first completed genome for a human-associated TM7 phylotype revealed a complete lack of amino acid biosynthetic capacity, and comparative genomics analyses with uncultivated environmental TM7 assemblies show remarkable conserved gene synteny and only minimal gene loss/gain that may have occurred as TM7x adapted to conditions within the human host.

TL;DR: An approach for increasing throughput and effectively improving the likelihood of sampling rare events based on forming small random pools of single-flow–sorted cells, amplifying their DNA by multiple displacement amplification and sequencing all cells in the pool, creating a “mini-metagenome” is developed.

TL;DR: This study demonstrates that it now has the capability to grow stable oral microbial in vitro biofilms containing more than one hundred operational taxonomic units (OTU) which represent 60-80% of the original inoculum OTU richness.

TL;DR: This study focuses on the recovery of a nearly complete genome representing a novel strain of the periodontal pathogen Porphyromonas gingivalis using the single-cell assembly tool SPAdes and shows for the first time that it enables comparative genomic analysis of strain variation in a pathogen captured from complex biofilm samples in a healthcare facility.

TL;DR: For the maintenance of healthy plaque pH, gene transcription activity of known and previously unrecognized pH-neutralizing pathways was associated with the genera Lactobacillus, Veillonella and Streptococcus during the pH recovery phase.