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Adam R. Karpf
Researcher at University of Nebraska Medical Center
Publications - 111
Citations - 7281
Adam R. Karpf is an academic researcher from University of Nebraska Medical Center. The author has contributed to research in topics: DNA methylation & Cancer. The author has an hindex of 42, co-authored 107 publications receiving 6488 citations. Previous affiliations of Adam R. Karpf include Eppley Institute for Research in Cancer and Allied Diseases & University of Texas at Austin.
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Journal ArticleDOI
DNA Demethylation in Zebrafish Involves the Coupling of a Deaminase, a Glycosylase, and Gadd45
TL;DR: Using zebrafish embryos, evidence is provided for a coupled mechanism of 5-meC demethylation, whereby AID deaminates 5- meC, followed by thymine base excision by Mbd4, promoted by Gadd45.
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Direct interaction between DNMT1 and G9a coordinates DNA and histone methylation during replication
Pierre-Olivier Estève,Hang Gyeong Chin,Andrea Smallwood,George R. Feehery,Omkaram Gangisetty,Adam R. Karpf,Michael Carey,Sriharsa Pradhan +7 more
TL;DR: Direct cooperation between DNMT1 and G9a provides a mechanism of coordinated DNA and H3K9 methylation during cell division and led to enhanced DNA and histone methylation of in vitro assembled chromatin substrates.
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Mutations in DNMT1 cause hereditary sensory neuropathy with dementia and hearing loss
Christopher J. Klein,Maria Victoria Botuyan,Yanhong Wu,Christopher J. Ward,Garth A. Nicholson,Simon Hammans,Kaori Hojo,Hiromitch Yamanishi,Adam R. Karpf,Douglas C. Wallace,Mariella Simon,Cecilie M. Lander,Lisa A. Boardman,Julie M. Cunningham,Glenn E. Smith,William J. Litchy,Benjamin Boes,Elizabeth J. Atkinson,Sumit Middha,P. James B. Dyck,Joseph E. Parisi,Georges Mer,David I. Smith,Peter J. Dyck +23 more
TL;DR: These mutations cause premature degradation of mutant proteins, reduced methyltransferase activity and impaired heterochromatin binding during the G2 cell cycle phase leading to global hypomethylation and site-specific hypermethylation.
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Reactivating the expression of methylation silenced genes in human cancer.
Adam R. Karpf,David A. Jones +1 more
TL;DR: The mechanisms of gene silencing by DNA methylation, the techniques used to decipher the complement of methylation-inactivated genes in human cancers, and current and future strategies for reactivating the expression of methylisation-silenced genes are discussed.
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Genetic disruption of cytosine DNA methyltransferase enzymes induces chromosomal instability in human cancer cells.
Adam R. Karpf,Sei Ichi Matsui +1 more
TL;DR: It is shown that DNMT deficiency in human cancer cells results in constitutive genomic instability manifested by chromosomal translocations, meeting the formal definition of genomic instability.