Alex J. Wolf

TL;DR: A genetic screen to identify genes whose overexpression disrupts telomeric silencing isolated 10 DOT genes, which include both components and regulators of silent chromatin, suggesting that DOT1 and DOT4 normally play important roles in gene repression.

TL;DR: Unexpectedly, overproduction of the RAP1 protein was also shown to decrease greatly chromosome stability, suggesting that R AP1 mediates interactions that have a more global effect on chromosome behavior than simply protecting telomeres from degradation.

TL;DR: Southern hybridization data provide evidence for a cell cycle-dependent change in telomere structure and demonstrate that TG1-3 tails, generated during replication of a linear plasmid in vivo, are capable of mediating telomeres-telomere interactions.

TL;DR: Density transfer experiments established that the acquisition of telomeric TG1-3 single-strand tails occurred immediately after the replication forks approached the ends of YLpFAT10, indicating that telomere replication may be the very last step in S phase.

TL;DR: A genetic screen to isolate mutant alleles of the histones H3 and H4 genes that would “lock” telomeric marker genes into a silenced state discovered that each of these mutants had a dramatic reduction in the level of acetylation at lysine 12 within the histone H4 tail, which is proposed to serve as a “memory mark” for propagating the expression state of a telomersic gene.