Showing papers by "Alexander Rebl published in 2012"
TL;DR: A first comparison of gene expression profiles in liver tissue of healthy rainbow trout from the local selection strain BORN and imported trout revealed a comparably faster regeneration of LEAP2A mRNA abundance in BORN trout following bacterial infection, indicating that the examined genes contribute to an improved first barrier against invading pathogens.
18 citations
TL;DR: The cytogenetic assignments in BBUX, BTAX, and OARX represent useable anchors for the ongoing genome sequence assembly in Bovidae and confirm and refine syntenic fragments in HSAX and identify several evolutionary breakpoints between the compared chromosomes.
Abstract: Based on a recently generated comprehensive gene map for Ovis aries chromosome X (OARX) with an approximately even locus distribution, we assigned selected bacterial artificial chromosome (BAC) probes corresponding to these OARX loci to Bubalus bubalis (BBU) and Bos taurus (BTA) by comparative fluorescence in-situ hybridization (FISH) to improve cytogenetically the X chromosome maps in these species. Twenty-five added loci in BBUX and BTAX, respectively, contribute to a more detailed description of the cytogenetic organization of these chromosomes. Further seven loci were identified in OARX and two DNA probes were assigned to X and Y chromosomes in river buffalo, cattle, and sheep, respectively, and thus identified loci in the pseudoautosomal region. The additional assignments double the number of cytogenetic loci in BBUX and increase their number in BTAX and OARX. The larger quantity of cytogenetic anchors allows a more precise morphological comparison of bovid X chromosomes among each other and with the Homo sapiens (HSA) X chromosome. The anchor loci confirm and refine syntenic fragments in HSAX and identify several evolutionary breakpoints between the compared chromosomes. The cytogenetic assignments in BBUX, BTAX, and OARX represent useable anchors for the ongoing genome sequence assembly in Bovidae.
14 citations
TL;DR: Comparative transcriptome analysis in healthy fish of two aquacultured rainbow trout lines varying in susceptibility to environmental stress identified significant differences in the expression of the Ca2+-binding protein regucalcin (RGN).
Abstract: The Ca2+-binding protein regucalcin (RGN) is crucial for the regulation of Ca2+ ion homeostasis and signal transduction of cells. It is involved in the regulation of Ca2+-dependent protein kinases and Ca2+ pump enzymes in cell membranes. Comparative transcriptome analysis in healthy fish of two aquacultured rainbow trout (Oncorhynchus mykiss) lines (BORN, TCO) varying in susceptibility to environmental stress identified significant differences in the expression of the RGN gene. Therefore, we firstly determined the full genomic DNA and cDNA sequence of RGN gene from rainbow trout and comparatively investigated the complete cDNA sequence in another salmonid fish dedicated for local aquaculture, the maraena whitefish (Coregonus marena). The sequence coding region translates for proteins of 298 and 299 amino acids (aa), respectively, indicating a high conservation of RGN proteins (95.7% aa identity) between the two related salmonids. In the second place, we generated RGN gene expression profiles after pathogen (Aeromonas salmonicidae subsp. salmonicida) and temperature (8 and 23°C) challenge in the two rainbow trout lines using salmon microarrays and quantitative RT-PCR. The profiles not only verified initially detected gene expression differences, they also display a tissue specific gene expression in dependence from the stressor and time. The differences in gene expression support our assumption that RGN might play a role in recovery of rainbow trout after environmental stress.
11 citations
TL;DR: Phylogenetic analyses suggest that bony fish possess two NELL genes, though these are either present as NELL1/2 pair corresponding to their mammalian orthologs or as a NELL2a/2b combination that might have arisen by a fish-specific duplication event.
Abstract: Mammalian neural epidermal growth factor-like-like 1 and 2 genes (NELL1 and NELL2) encode multifunctional glycoproteins involved in cell growth regulation and differentiation. We isolated two closely related NELL2 transcript sequences from rainbow trout (Oncorhynchus mykiss). These conceptually translated NELL2a and -b sequences share 83% identical residues and the NELL-typical structure. Phylogenetic analyses suggest that bony fish possess two NELL genes, though these are either present as NELL1/2 pair corresponding to their mammalian orthologs or as a NELL2a/2b combination that might have arisen by a fish-specific duplication event. Both trout NELL2 genes are highly expressed at early developmental stages. In adult rainbow trout, NELL2a copies were detected in each tissue analyzed, whereas NELL2b is abundantly expressed only in brain. Cerebral NELL2a/b gene expression seems to be temperature-independent, whereas NELL2a gene expression is clearly down-regulated in gill and up-regulated in muscle tissue after temperature elevation. Infection with Aeromonas salmonicida leads to a considerable increase in NELL2a/b copy number in trunk kidney at day 7 p.i. Moreover, comparative qRT-PCR revealed different NELL2a/b expression pattern in two rainbow trout strains, imported rainbow trout TCO and a local trout selection strain BORN that is known to be resistant to several biotic and abiotic stressors.
4 citations