Showing papers by "André Bensadoun published in 1994"
TL;DR: It is demonstrated that HL functions in the metabolism of HDL and IDL, thereby playing a key role in plasma cholesterol homeostasis.
Abstract: To elucidate the precise metabolic roles of hepatic lipase (HL), a human HL cDNA in a liver-specific expression vector was used to generate transgenic lines in the rabbit, an animal that normally expresses low levels of this enzyme. HL was detected in the plasma of all rabbits only after the administration of heparin; HL activity in transgenic rabbits was found at levels up to 80-fold greater than that in nontransgenic littermates. This increase in enzyme activity was associated with as much as a 5-fold decrease in total plasma cholesterol levels. Expression of the transgene resulted in a dramatic reduction in the level of large high density lipoproteins (HDL1 and HDL2) as well as dense HDL3. A reduction in the quantity of intermediate density lipoproteins (IDL) was also observed. These results demonstrate that HL functions in the metabolism of HDL and IDL, thereby playing a key role in plasma cholesterol homeostasis.
228 citations
TL;DR: It is concluded that hepatic lipase facilitates, but is not essential for, the initial uptake of chylomicron remnants by rat liver, and that endocytosis of chyomaticron remnants does not require binding to hepaticlipase or the associated hydrolysis of remnant lipids.
144 citations
TL;DR: Rat hepatoma McA-RH7777 cells transfected with a human hepatic lipase cDNA synthesized and secreted 50-80 ng of human HL/mg of cell protein at 4 h, approximately 50% of which was bound to cell-surface heparan sulfate proteoglycans (HSPG).
140 citations
TL;DR: The heterogeneity of core proteins of HSPGs with affinity for LPL may provide a structural basis for the multiple fates of LPL on the surface of adipocytes, i.e. internalization, degradation, or recycling to the cell surface and translocation into the medium.
29 citations
1 citations
1 citations