Andrea Niederwieser

TL;DR: This work shows that ManNAc derivatives containing a terminal alkene in the acyl side chain are metabolically incorporated into cell-surface sialic acids and can subsequently be labeled by the DARinv, and identifies monosubstituted (terminal) alkenes as a new class of chemical reporters.

TL;DR: It is demonstrated that the cell's biosynthetic machinery incorporates this non-natural mannosamine derivative into glycoconjugates that can, subsequently, be labeled within less than 10 min with a new sulfo-Cy3-tetrazine conjugate.

TL;DR: This work presents an approach for the postsynthetic labelling of enzymatically synthesized vinyl-modified DNA by Diels-Alder reaction with inverse electron demand using a tetrazine that proceeds very efficiently and supersedes several known approaches.

TL;DR: In this article, the authors examined the Diels-Alder reaction with inverse-electron-demand (DARinv) as an irreversible, chemoselective ligation reaction for the preparation of carbohydrate arrays.

TL;DR: Kinetic investigations showed that the reactivity of the alkenes in DAinv reactions increases with growing chain length, and one of the compounds, peracetylated N-butenyloxycarbonylmannosamine, was especially well suited for labeling cell-surface glycans and represents the optimal balance between incorporation efficiency and speed of the labeling reaction.