O
Olga Abian
Researcher at University of Zaragoza
Publications - 129
Citations - 4415
Olga Abian is an academic researcher from University of Zaragoza. The author has contributed to research in topics: Isothermal titration calorimetry & Chemistry. The author has an hindex of 28, co-authored 113 publications receiving 3676 citations. Previous affiliations of Olga Abian include University of Barcelona & International Association of Classification Societies.
Papers
More filters
Journal ArticleDOI
Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment☆
TL;DR: Progressive establishment of new enzyme-support attachments was showed by the progressive irreversible covalent immobilization of several subunits of multi-subunits proteins, and the final stabilization factor become 100-fold comparing soluble penicillin G acylase and optimal derivative.
Journal ArticleDOI
Multifunctional epoxy supports: a new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage.
TL;DR: Multifunctional supports containing epoxy groups are proposed as a second generation of activated supports for covalent immobilization of enzymes following the epoxy chemistry on any type of support (hydrophobic or hydrophilic ones) under very mild experimental conditions.
Journal ArticleDOI
Epoxy sepabeads: a novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment.
Cesar Mateo,Olga Abian,Gloria Fernández-Lorente,Justo Pedroche,Roberto Fernandez-Lafuente,Jose M. Guisan,Andrea Tam,Moreno Daminati +7 more
TL;DR: Sepabeads‐EP (a new epoxy support) has been utilized to immobilize‐stabilize the enzyme penicillin G acylase via multipoint covalent attachment and was hundreds‐fold more stable than Eupergit C derivatives when using a more sophisticated three‐step immobilization/stabilization/blockage procedure.
Journal ArticleDOI
Some special features of glyoxyl supports to immobilize proteins
Cesar Mateo,Olga Abian,Marieta Bernedo,Emma Cuenca,Manuel Fuentes,Gloria Fernández-Lorente,Jose M. Palomo,Valeria Grazú,Benevides C. C. Pessela,Cecilia Giacomini,Cecilia Giacomini,Gabriela Irazoqui,Gabriela Irazoqui,Andrea Villarino,Andrea Villarino,Karen Ovsejevi,Karen Ovsejevi,Francisco Batista-Viera,Roberto Fernandez-Lafuente,Jose M. Guisan +19 more
TL;DR: This mechanism promotes that proteins become immobilized by the area/s where the highest lysine residues density is located and explain the high enzyme stabilization usually achieved by using this immobilization technique.
Journal ArticleDOI
Epoxy-Amino Groups: A New Tool for Improved Immobilization of Proteins by the Epoxy Method
Cesar Mateo,Rodrigo Torres,Gloria Fernández-Lorente,Claudia Ortiz,Manuel Fuentes,Aurelio Hidalgo,Fernando López-Gallego,Olga Abian,Jose M. Palomo,Lorena Betancor,Benevides C. C. Pessela,Jose M. Guisan,Roberto Fernandez-Lafuente +12 more
TL;DR: Stability of the immobilized enzyme has been found to be much higher using the new support than in preparations using the conventional ones in many cases, and immobilization is much more rapid using amino-epoxy supports than employing conventional epoxy supports.