다중혈관 관상동맥 환자에서 y-문합을 이용하여 양쪽 내흉동맥만을 사용한 우회술의 조기 성적

The human genome holds an extraordinary trove of information about human development, physiology, medicine and evolution. Here we report the results of an international collaboration to produce and make freely available a draft sequence of the human genome. We also present an initial analysis of the data, describing some of the insights that can be gleaned from the sequence.

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Initial sequencing and analysis of the human genome.

Statistical method for testing the neutral mutation hypothesis by DNA polymorphism.

So far in this course we have dealt entirely with the evolution of characters that are controlled by simple Mendelian inheritance at a single locus. There are notes on the course website about gametic disequilibrium and how allele frequencies change at two loci simultaneously, but we didn’t discuss them. In every example we’ve considered we’ve imagined that we could understand something about evolution by examining the evolution of a single gene. That’s the domain of classical population genetics. For the next few weeks we’re going to be exploring a field that’s actually older than classical population genetics, although the approach we’ll be taking to it involves the use of population genetic machinery. If you know a little about the history of evolutionary biology, you may know that after the rediscovery of Mendel’s work in 1900 there was a heated debate between the “biometricians” (e.g., Galton and Pearson) and the “Mendelians” (e.g., de Vries, Correns, Bateson, and Morgan). Biometricians asserted that the really important variation in evolution didn’t follow Mendelian rules. Height, weight, skin color, and similar traits seemed to

Human biochemical genetics

Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants

Both disease and treatment of the cornea alter its microstructural and biomechanical properties. Previous work showed that dynamic light scattering (DLS) reflects changes in the cornea with respect to crosslinking treatment and post-surgical healing in vivo. However, due to the complex structure of the corneal stroma, the exact mechanisms which give rise to the DLS signal are unclear. This work attempts to determine the cause of DLS in the cornea by comparing the scattering signal under different conditions. The conclusions drawn from these studies inform the use of OCT-based dynamic light scattering measurements for corneal assessment. 

Investigating corneal properties through temporal decorrelation in OCT images (Conference Presentation)

Purpose The purpose of this work is to determine the sensitivity of phase-decorrelation optical coherence tomography (OCT) to protein aggregation associated with cataracts in the ocular lens, as compared to OCT signal intensity. Methods Six fresh porcine globes were held at 4°C until cold cataracts developed. As the globes were re-warmed to ambient temperature, reversing the cold cataract, each lens was imaged repeatedly using a conventional OCT system. Throughout each experiment, the internal temperature of the globe was recorded using a needle-mounted thermocouple. OCT scans were acquired, their temporal fluctuations were analyzed, and the rates of decorrelation were spatially mapped. Both decorrelation and intensity were evaluated as a function of recorded temperature. Results Both signal decorrelation and intensity were found to change with lens temperature, a surrogate of protein aggregation. However, the relationship between signal intensity and temperature was not consistent across different samples. In contrast, the relationship between decorrelation and temperature was found to be consistent across samples. Conclusions In this study, signal decorrelation was shown to be a more repeatable metric for quantification of crystallin protein aggregation in the ocular lens than OCT intensity-based metrics. Thus, OCT signal decorrelation measurements could enable more detailed and sensitive study of methods to prevent cataract formation. Translational Relevance This dynamic light scattering-based approach to early cataract assessment can be implemented on existing clinical OCT systems without hardware additions, so it could quickly become part of a clinical study workflow or an indication for use for a pharmaceutical cataract intervention.

Phase-Decorrelation Optical Coherence Tomography Measurement of Cold-Induced Nuclear Cataract

Genetic maps describe the order of polymorphisms along a chromosome; however, unlike physical maps, the map distances are related to recombination. The level of recombination varies with position along the chromosome and between the sexes. It is important to integrate genetic and physical or sequence-based maps to identify regions of elevated or reduced recombination. The presence of recombination hot and cold spots has a bearing on efforts to map disease genes and for the understanding of the biological properties of chromosome location.


Keywords:

linkage mapping;
recombination;
interference;
map integration

Genetic Maps: Integration

A new mitomycin C (MM(Z)-sensitive rodent line, UV40. has been identified in the collection of ultraviolet light- (UV-) sensitive mutants of Chinese hamster ovary (CHO) cells isolated at the previous Facility for Automated Experiments in Cell Biology (FAECB). It was isolated from an UV mutant hunt using mutagenesis of AA8 cells with the DNA intercalating frameshift mutagen ICR 170. It is complemented by CHO-UV- I, irs I, irs3, irsl SF. MC.5, V-C8 and V-H4 with respect to its MMC sensitivity based on cell survival. Despite having approx. 4 X normal UV sensitivity and increased sensitivity to UV inhibition of DNA replication, it has near-normal incision kinetics of UV irradiated DNA, and normal (6-4) photoproducts removal. It also is not hypermutable by UV, and shows near normal levels of UV inhibition of RNA synthesis. UV40 also has approx. I I X , IO X , 5 X and 2 X AA8 sensitivity to MMC, ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), and X-rays, respectively. Thus, its defect apparently does not involve nucleotide excision repair but rather another process, possibly in replicating past lesions. The spontaneous chromosomal aberration frequency is elevated to 20% in

/pdf/a-cho-mutant-uv40-that-is-sensitive-to-diverse-mutagens-and-rfqyhpr4le.pdf

Andrew Collins

Papers

Investigating corneal properties through temporal decorrelation in OCT images (Conference Presentation)

Phase-Decorrelation Optical Coherence Tomography Measurement of Cold-Induced Nuclear Cataract

Genetic Maps: Integration

A CHO mutant, UV40, that is sensitive to diverse mutagens and represents a new complementation sensitivity group of mitomycin C

Candidate Gene (Candidate Gene‐based Analysis)