Andrew D. Huber

TL;DR: The context of template sequence defines the relative contribution of each mechanism and affects the affinity of EFdA-MP for potential incorporation sites, explaining in part the lack of antagonism between EFd

TL;DR: A branched DNA in situ hybridization method for direct single-cell visualization of HIV DNA, RNA, and protein, which can be used to simultaneously detect multiple viral nucleic acid intermediates, characterize the effects of host factors or drugs on steps of the HIV life cycle, or its reactivation from the latent state, thus facilitating the development of antivirals and latency reactivating agents.

TL;DR: The design, synthesis and biological evaluation of a novel N-hydroxy thienopyrimidine-2,3-dione chemotype (11) which potently and selectively inhibited RNase H with considerable potency against HIV-1 in cell culture is reported.

TL;DR: In vitro ADME assays demonstrated that this novel chemotype possesses largely favorable physicochemical properties suitable for further development, and biochemical testing and molecular modeling strongly corroborate the inhibition of INST as the antiviral mechanism of action.

TL;DR: A critical redesign of the HPD chemotype featuring an additional wing at the C5 position that led to drastically improved RNase H inhibition and significant antiviral activity is reported.