Anna Casasent

TL;DR: A highly multiplexed single-nucleus sequencing method is developed to investigate copy number evolution in patients with triple-negative breast cancer, showing that the majority of copy number aberrations are acquired at the earliest stages of tumor evolution, in short punctuated bursts that form the tumor mass.

TL;DR: Topographic Single Cell Sequencing data reveal a direct genomic lineage between in situ and invasive tumor subpopulations and further show that most mutations and copy number aberrations evolved within the ducts prior to invasion.

TL;DR: A highly multiplexed single-cell DNA sequencing approach was developed to trace the metastatic lineages of two CRC patients with matched liver metastases and revealed an unexpected independent tumor lineage that did not metastasize, and early progenitor clones with the "first hit" mutation in APC that subsequently gave rise to both the primary and metastatic tumors.

TL;DR: In this article, a single-cell, single-molecule DNA-sequencing method was used to investigate copy number evolution during the expansion of primary breast tumours and showed that triple-negative breast cancers continue to evolve chromosome aberrations and maintain a reservoir of subclonal diversity during primary tumour growth.

TL;DR: The role of intratumour heterogeneity in the progression of DCIS to IDC is discussed in the context of three evolutionary models: independent lineages, evolutionary bottlenecks, and multiclonal invasion.