Anne Bremer

TL;DR: It is shown that the numbers (valence) of aromatic residues in PLDs determine the extent of temperature-dependent compaction of individual molecules in dilute solutions, which determines full binodals that quantify concentrations of PLDs within coexisting dilute and dense phases as a function of temperature.

TL;DR: In this paper, a conceptual framework that divides IDRs into interacting and solvating regions has been proposed, and analytical instantiations and coarse-grained models can test their understanding of the driving forces against experimental phase behavior.

TL;DR: Validated simulation paradigms enable the exploration of sequence space to help the understanding of how disordered protein regions can encode phase behavior, which IDRs may mediate phase separation in cells, and which IDR are in contrast highly soluble.

TL;DR: Two truncated versions of LEA25 are created (2H and 4H) to elucidate the structural and functional significance of this domain, which was important in cryoprotection as the central domain itself only provided a low level of protection.

TL;DR: It is shown that COR15A starts to fold into α-helices already under mild dehydration conditions (97% relative humidity (RH), corresponding to freezing at -3°C) and that folding gradually increases with decreasing RH, in agreement with the hypothesis that Cor15A-membrane interaction is at least, in part, driven by a hydrophobic interaction between the lipids and thehydrophobic face of the amphipathic protein α-helix.