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Annette S. Uss
Researcher at Schering-Plough
Publications - 15
Citations - 698
Annette S. Uss is an academic researcher from Schering-Plough. The author has contributed to research in topics: RNA-dependent RNA polymerase & RNA. The author has an hindex of 11, co-authored 15 publications receiving 664 citations.
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Journal ArticleDOI
De Novo Initiation of RNA Synthesis by Hepatitis C Virus Nonstructural Protein 5B Polymerase
TL;DR: It was demonstrated that a recombinant HCV NS5B protein has the ability to initiate de novo RNA synthesis in vitro and preferred GTP as the initiation nucleotide, which can be labeled by [γ-32P]nucleoside triphosphate.
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Mutational Analysis of Bovine Viral Diarrhea Virus RNA-Dependent RNA Polymerase
Vicky C. H. Lai,C. Cheng Kao,Eric Ferrari,Justin J. Park,Annette S. Uss,Jacquelyn Wright-Minogue,Zhi Hong,Johnson Y. N. Lau +7 more
TL;DR: Recombinant bovine viral diarrhea virus (BVDV) nonstructural protein 5B (NS5B) produced in insect cells has been shown to possess an RNA-dependent RNA polymerase (RdRp) activity and was found to utilize a circular single-stranded DNA as a template for RNA synthesis, suggesting that synthesis does not require ends in the template.
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Prediction of oral drug absorption in humans--from cultured cell lines and experimental animals.
TL;DR: Since it is well recognized that human intestinal absorption cannot be precisely predicted by a single screening assay, it is important to utilize various in-vitro and in- vivo non-clinical studies during lead optimization in drug discovery.
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Identification of transcription factor binding sites important in the regulation of the human interleukin-5 gene.
Kimberly S. Stranick,Demetris N. Zambas,Annette S. Uss,Robert W. Egan,M. Motasim Billah,Shelby P. Umland +5 more
TL;DR: The sequence involved in stimulation-dependent transcription is defined and constitutive as well as inducible DNA-binding protein complexes that bind to RE-II are identified, which is shown to be critical for inducibles IL-5 promoter activity in transient transfection assays.
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Development of in vitro pharmacokinetic screens using Caco-2, human hepatocyte, and Caco-2/human hepatocyte hybrid systems for the prediction of oral bioavailability in humans.
TL;DR: This study demonstrates that the Caco-2/hepatocyte hybrid model is more favorable and further proves the potential and feasibility of using in vitro screenings for the prediction of in vivo pharmacokinetics in humans.