Antonio Di Giulio

TL;DR: This work investigates the antibacterial, haemolytic and cytotoxic activities of temporin L (FVQWFSKFLGRIL-NH2), isolated from the skin of the European red frog Rana temporaria, which displayed the highest activity of temporins studied to date, against both human erythrocytes and bacterial and fungal strains.

TL;DR: The results indicate that the lytic activity of temporins is not greatly affected by the membrane composition; temporins A and B allow the leakage of large-size molecules from the bacterial cells; temporin H renders both the outer and inner membrane of bacteria permeable to hydrophobic substances of low molecular mass; and temporin D, although devoid of antibacterial activity, has a cytotoxic effect on erythrocytes.

TL;DR: The results reveal that temporin L increases the permeability of the bacterial inner membrane in a dose-dependent manner without destroying the cell's integrity, in contrast with many antimicrobial peptides.

TL;DR: The activity of temporin L when combined with imipenem and piperacillin in the prevention of lethality in two rat models of septic shock is characterized, measuring bacterial growth in blood and intra-abdominal fluid, endotoxin and tumor necrosis factor alpha concentrations in plasma, and lethality.

TL;DR: Temporins are short and linear antimicrobial peptides first isolated from the skin of the European red frog, Rana temporaria, and are effective against Gram-positive bacteria and Candida albicans and in the presence of the common bacterial negatively charged phospholipid phosphatidylglycerol.