A
Artur Scherf
Researcher at Pasteur Institute
Publications - 168
Citations - 11808
Artur Scherf is an academic researcher from Pasteur Institute. The author has contributed to research in topics: Plasmodium falciparum & Gene. The author has an hindex of 53, co-authored 158 publications receiving 10962 citations. Previous affiliations of Artur Scherf include Centre national de la recherche scientifique & National Autonomous University of Mexico.
Papers
More filters
Journal ArticleDOI
Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system
Mehdi Ghorbal,Molly Gorman,Cameron Ross MacPherson,Rafael M. Martins,Artur Scherf,Jose-Juan Lopez-Rubio +5 more
TL;DR: The CRISPR-Cas9 system was demonstrated for use in P. falciparum by disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions with high efficiency, and an artemisinin-resistant strain was generated, illustrating the value of efficient genome editing in malaria research.
Journal ArticleDOI
Antigenic variation in malaria: in situ switching, relaxed and mutually exclusive transcription of var genes during intra‐erythrocytic development in Plasmodium falciparum
Artur Scherf,Rosaura Hernández-Rivas,Pierre Buffet,Emmanuel Bottius,C. Benatar,B. Pouvelle,Jürg Gysin,Michael Lanzer +7 more
TL;DR: The results suggest that an epigenetic mechanism(s) is involved in var gene regulation, and are likely mediated at the level of transcriptional initiation, as demonstrated by nuclear run‐on analyses.
Journal ArticleDOI
Frequent ectopic recombination of virulence factor genes in telomeric chromosome clusters of P. falciparum
Lucio H. Freitas-Junior,Emmanuel Bottius,Lindsay A. Pirrit,Kirk W. Deitsch,Christine Scheidig,Françoise Guinet,Françoise Guinet,Ulf Nehrbass,Thomas E. Wellems,Artur Scherf +9 more
TL;DR: In this paper, the authors identify a mechanism by which var gene sequences undergo recombination at frequencies much higher than those expected from homologous crossover events alone, and propose that the alignment of var genes in heterologous chromosomes facilitates gene conversion and promotes the diversity of antigenic and adhesive phenotypes.
Journal ArticleDOI
Antigenic Variation in Plasmodium falciparum
TL;DR: Key regulatory mechanisms thought to be critical for monoallelic expression of var genes are highlighted and controversies surrounding var genetic elements in antigenic variation are discussed.
Journal ArticleDOI
Telomeric Heterochromatin Propagation and Histone Acetylation Control Mutually Exclusive Expression of Antigenic Variation Genes in Malaria Parasites
Lucio H. Freitas-Junior,Rosaura Hernández-Rivas,Stuart A. Ralph,Dvorak Montiel-Condado,Omar K. Ruvalcaba-Salazar,Ana Paola Rojas-Meza,Liliana Mâncio-Silva,Ricardo J. Leal-Silvestre,Alisson M. Gontijo,Spencer L. Shorte,Artur Scherf +10 more
TL;DR: The data demonstrate that mutually exclusive transcription of var genes is linked to the dynamic remodeling of chromatin, and this work identifies chromatin modifications at telomeres that spread far into telomere-proximal regions, including var gene loci (>50 kb).