Showing papers by "Badri N. Pandey published in 2018"

Synthesis and characterization of monodispersed water dispersible Fe 3 O 4 nanoparticles and in vitro studies on human breast carcinoma cell line under hyperthermia condition

Abstract: Monodispersed Fe3O4 magnetic nanoparticles (MNPs) having size of 7 nm have been prepared from iron oleate and made water dispersible by functionalization for biomedical applications. Three different reactions employing thioglycolic acid, aspartic acid and aminophosphonate were performed on oleic acid coated Fe3O4. In order to achieve a control on particle size, the pristine nanoparticles were heated in presence of ferric oleate which led to increase in size from 7 to 11 nm. Reaction parameters such as rate of heating, reaction temperature and duration of heating have been studied. Shape of particles was found to change from spherical to cuboid. The cuboid shape in turn enhances magneto-crystalline anisotropy (Ku). Heating efficacy of these nanoparticles for hyperthermia was also evaluated for different shapes and sizes. We demonstrate heat generation from these MNPs for hyperthermia application under alternating current (AC) magnetic field and optimized heating efficiency by controlling morphology of particles. We have also studied intra-cellular uptake and localization of nanoparticles and cytotoxicity under AC magnetic field in human breast carcinoma cell line.

Covalent immobilization of doxorubicin in glycine functionalized hydroxyapatite nanoparticles for pH-responsive release

Abstract: In this study, amino acid (glycine) functionalized hydroxyapatite nanoparticles (Gly-HANPs) were synthesized in situ by a simple co-precipitation method. The structural and morphological properties of the synthesized nanoparticles were evaluated using various characterization techniques including powder X-ray diffraction and electron microscopy. The presence of glycine functionalities on the surface of the nanoparticles was ascertained from infrared spectroscopy, thermal analysis, CHN analysis and zeta potential measurements. Doxorubicin (DOX), an anthracycline antibiotic commonly used in cancer chemotherapy, was covalently bound to Gly-HANPs via a pH sensitive imine linkage using glutaraldehyde as a cross-linker. The DOX loaded nanoparticles (DOX-Glut-Gly-HANPs) showed pH-responsive controlled and sustained release of the drug. In particular, the drug release rate from nanoparticles was higher under acidic pH conditions, characteristic of tumour tissues, than at physiological pH, typical for normal tissues. The higher release of DOX at acidic pH is indicative of the cleavage of the imine (–CN–) linkage at acidic pH. The cell uptake and therapeutic efficacy of DOX conjugated nanoparticles were investigated in WEHI-164 mouse fibrosarcoma cancer cells. The in vitro cytotoxicity studies suggested that glutaraldehyde treated nanoparticles (Glut-Gly-HANPs) have insignificant cytotoxicity towards WEHI-164 cancer cells, however the DOX loaded nanoparticles exhibited significant dose and time dependent cytotoxicity towards WEHI-164 cells. Furthermore, the confocal laser scanning microscopy studies demonstrated good cellular uptake of the DOX loaded nanoparticles into the WEHI-164 cells. The obtained results suggested that these nanoparticles can serve as potential carriers for the pH-triggered delivery of an anticancer drug.

Receptor tyrosine kinase signaling in cancer radiotherapy and its targeting for tumor radiosensitization.

Abstract: Purpose: One of the most important implications of ‘Radiation Biology’ research is to improve cancer radiotherapy with minimum side effects. In this regard, combination of chemotherapy with...

Thorium-induced Anatomical and Histopathological Changes in Liver of Swiss Mice

Abstract: Present study is aimed to investigate the anatomical and histopathological changes in liver tissues of Swiss albino male mice, one month after intravenous administration of thorium (232Th; 4 and 40 mg/kg). Synchrotron X-ray micro-CT imaging, CD31 immuno-cytochemistry, hematoxylin & eosin staining and Periodic acid-Schiff staining were performed to study changes in liver anatomy, blood vessels/capillaries, histology and glycogen content of liver, respectively. Synchrotron X-ray micro-CT imaging of liver showed loss of blood vessels in mice treated with thorium (4 mg/kg), which was more prominent at higher dose of thorium (40 mg/kg). These thorium-induced changes in liver were correlated with the decrease in CD31 positive cells and loss of tissue architecture. A dose-dependent increase in glycogen content was also observed in the liver of thorium-treated mice. Our results provide novel insight about the effects of thorium on liver, which may have significant implications in understanding the mechanism of thorium thorium-induced hepatotoxicity.

Biomarkers in chronic obstructive pulmonary disease patients for prediction of lung cancer development

Abstract: According to the World Health Organization (2016), chronic obstructive pulmonary disease (COPD) and lung cancer (along with trachea and bronchial cancers) are third and sixth among 10 top causes of death globally. The association between lung cancer and COPD has been widely established owing to their common endogenous and exogenous risk factors. Mechanistically, lung cancer and COPD are interlinked diseases in many ways such as oxidative stress-associated DNA damage, inflammation, and telomere shortening. An increase in lung cancer has been well correlated with smoking, which is likely to occur up to five folds higher in smokers with COPD than normal lung function subjects. In majority of cases, lung cancer development, especially in COPD patients, is asymptomatic and only diagnosed at advanced stages with poor prognosis. The development of biomarkers for early prediction of lung cancer in both high- and low-risk COPD patients will help clinicians for their better follow-up, early diagnosis, and improved therapeutic management.