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Benjamin Steyer
Researcher at University of Wisconsin-Madison
Publications - 11
Citations - 292
Benjamin Steyer is an academic researcher from University of Wisconsin-Madison. The author has contributed to research in topics: Gene & Induced pluripotent stem cell. The author has an hindex of 6, co-authored 11 publications receiving 229 citations. Previous affiliations of Benjamin Steyer include Wisconsin Alumni Research Foundation & University of California, Riverside.
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Journal ArticleDOI
Electrochemical oxidation of ferrocene: a strong dependence on the concentration of the supporting electrolyte for nonpolar solvents.
Duoduo Bao,Brent Millare,Wei Xia,Benjamin Steyer,Alexander A. Gerasimenko,Amy Ferreira,Antonio Contreras,Valentine I. Vullev +7 more
TL;DR: The dependence of the oxidation potential of ferrocene on the electrolyte concentration for differentsolvents revealed that the abovementioned approximation in the Born correction term indeed introduces a significant error in the estimation of the charge-transfer driving force from redox data collected using relatively nonpolar solvents.
Journal ArticleDOI
Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection
Benjamin Steyer,Qian Bu,Evan Cory,Keer Jiang,Stella Duong,Divya Sinha,Stephanie S. Steltzer,David M. Gamm,Qiang Chang,Krishanu Saha +9 more
TL;DR: A robust method for rapid, scarless introduction or correction of disease-associated variants in hPSCs using CRISPR/Cas9 is presented and characterized.
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Human iPSC Modeling Reveals Mutation-Specific Responses to Gene Therapy in a Genotypically Diverse Dominant Maculopathy
Divya Sinha,Benjamin Steyer,Pawan K Shahi,Katherine P. Mueller,Rasa Valiauga,Kimberly L. Edwards,Cole Bacig,Stephanie S. Steltzer,Sandhya Srinivasan,Amr A. Abdeen,Evan Cory,Viswesh Periyasamy,Alireza Fotuhi Siahpirani,Edwin M. Stone,Budd A. Tucker,Sushmita Roy,Bikash R. Pattnaik,Krishanu Saha,David M. Gamm +18 more
TL;DR: The results suggest that gene augmentation is a viable first-line approach for some individuals with dominant Best disease and that non-responders are candidates for alternate approaches such as gene editing, however, testing gene editing strategies for on-target efficiency and off-target events using personalized iPSC-RPE model systems is warranted.
Journal ArticleDOI
High content analysis platform for optimization of lipid mediated CRISPR-Cas9 delivery strategies in human cells ☆
Benjamin Steyer,Jared Carlson-Stevermer,Nicolas Angenent-Mari,Andrew S. Khalil,Ty Harkness,Krishanu Saha +5 more
TL;DR: An innovative platform to generate and screen many formulations of synthetic biomaterials and components of the CRISPR-Cas9 system in parallel and identify formulation parameters for Cas9-material complexes that can optimize gene-editing in a specific human cell type is described.
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Drug-loaded nanoparticles induce gene expression in human pluripotent stem cell derivatives
Virendra Gajbhiye,Leah E. Escalante,Guojun Chen,Alex Laperle,Qifeng Zheng,Benjamin Steyer,Shaoqin Gong,Krishanu Saha +7 more
TL;DR: The results demonstrate that the PEG-H40-DXC nanoparticle system provides an effective tool to controlling gene expression in human stem cell derivatives and maintains higher fibroblast proliferation levels and MMP activity.